microcirculationРегионарное кровообращение и микроциркуляцияRegional blood circulation and microcirculation1682-66552712-9756Academician I.P. Pavlov First St. Petersburg State Medical University10.24884/1682-6655-2023-22-3-62-69microcirculation-1217Research ArticleОРИГИНАЛЬНЫЕ СТАТЬИ (КЛИНИЧЕСКИЕ ИССЛЕДОВАНИЯ)ORIGINAL ARTICLES (CLINICAL INVESTIGATIONS)Полиморфизм Arg72Pro (rs1042522) гена TР53 у пациентов с ишемической болезнью сердца – влияние на отдаленный прогноз после аортокоронарного шунтированияArg72Pro polymorphism (rs1042522) of the TP53 gene in patients with coronary artery disease: influence on long-term prognosis after coronary artery bypass graftinghttps://orcid.org/0009-0007-7895-0750МагамадовИ. С.MagamadovI. S.

Магамадов Иса Супьянович – врач-кардиолог отделения кардиохирургии

192242, Санкт-Петербург, ул. Будапештская, д. 3, литера А

Magamadov Isa S. – Cardiologist, Cardiosurgery Department

3, Budapeshtskaya str., Saint Petersburg, 192242 

isamagamadow17@gmail.comhttps://orcid.org/0000-0002-5017-0214СкородумоваЕ. А.SkorodumovaE. A.

Скородумова Елена Андреевна – д-р мед. наук, старший научный сотрудник, отдел неотложной кардиологии и ревматологии

192242, Санкт-Петербург, ул. Будапештская, д. 3, литера А

Skorodumova Elena A. – MD, Senior Researcher, Department of Emergency Cardiology and Rheumаtology

3, Budapeshtskaya str., Saint Petersburg, 192242 

elskor@mail.ruhttps://orcid.org/0000-0002-7015-1010КостенкоВ. А.KostenkoV. A.

Костенко Виктор Авенирович – д-р мед. наук, руководитель отдела неотложной кардиологии и ревматологии

92242, Санкт-Петербург, ул. Будапештская, д. 3, литера А

Kostenko Viktor A. – MD, Head, Department of Emergency Cardiology and Rheumаtology

3, Budapeshtskaya str., Saint Petersburg, 192242 

vic2012tor@gmail.comhttps://orcid.org/0000-0002-9492-4516ПивовароваЛ. П.PivovarovaL. P.

Пивоварова Людмила Павловна – д-р мед. наук, руководитель отдела лабораторной диагностики

92242, Санкт-Петербург, ул. Будапештская, д. 3, литера А

Pivovarova Lyudmila P. – MD, Head, Department of Laboratory Diagnostics

3, Budapeshtskaya str., Saint Petersburg, 192242 

pivovaroval@yandex.ruhttps://orcid.org/0000-0001-6311-1259АрискинаА. В.AriskinaO. B.

Арискина Ольга Борисовна – канд. биол. наук, научный сотрудник, отдел лабораторной диагностики

92242, Санкт-Петербург, ул. Будапештская, д. 3, литера А

Ariskina Olga B. – Candidate of Sciences (PhD) in Biology, Researcher, Department of Laboratory Diagnostics

3, Budapeshtskaya str., Saint Petersburg, 192242 

olga.ariskina@mail.ruhttps://orcid.org/0000-0002-6831-2153СиверинаА. В.SiverinaA. V.

Сиверина Анна Викторовна – младший научный сотрудник, отдел неотложной кардиологии и ревматологии

92242, Санкт-Петербург, ул. Будапештская, д. 3, литера А

Siverina Anna V. – Junior Researcher, Department of Emergency Cardiology and Rheumаtology

3, Budapeshtskaya str., Saint Petersburg, 192242 

gudkovanna_09@mail.ruhttps://orcid.org/0000-0002-4961-5570СкородумоваЕ. Г.SkorodumovaE. G.

Скородумова Елизавета Геннадьевна – младший научный сотрудник, отдел неотложной кардиологии и ревматологии

92242, Санкт-Петербург, ул. Будапештская, д. 3, литера А

Skorodumova Elizaveta G. – Junior Researcher, Department of Emergency Cardiology and Rheumаtology

3, Budapeshtskaya str., Saint Petersburg, 192242 

lisavetta91@mail.ruГосударственное бюджетное учреждение «Санкт-Петербургский научно-исследовательский институт скорой помощи имени И. И. Джанелидзе»РоссияDzhanelidze Research Institute of Emergency MedicineRussian Federation2023161020232236269Copyright © Магамадов И.С., Скородумова Е.А., Костенко В.А., Пивоварова Л.П., Арискина А.В., Сиверина А.В., Скородумова Е.Г., 20232023Магамадов И.С., Скородумова Е.А., Костенко В.А., Пивоварова Л.П., Арискина А.В., Сиверина А.В., Скородумова Е.Г.Magamadov I.S., Skorodumova E.A., Kostenko V.A., Pivovarova L.P., Ariskina O.B., Siverina A.V., Skorodumova E.G.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.microcirc.ru/jour/article/view/1217

Введение. Поиск генетических детерминант развития сердечно-сосудистых заболеваний занимает важное место в концепции персонализированной медицины. Цель. Оценить влияние полиморфизма Arg72Pro (rs1042522) гена ТP53 на отдаленный прогноз у пациентов c ишемической болезнью сердца (ИБС), подвергшихся аортокоронарному шунтированию (АКШ). Материалы и методы. В исследование было включено 89 пациентов со стабильной ИБС и многососудистым поражением коронарных артерий (КА). Первая выборка – 61 человек, с вариантами Аrg/Arg и Arg/Pro. Вторая – 28 человек с вариантом Pro/Pro. У пациентов оценивалось ремоделирование миокарда, качество жизни больных, а также клинические данные за 18 месяцев наблюдения. Результаты. У больных с вариантами Аrg/Arg и Arg/Pro гена ТР53 за период наблюдения произошло увеличение конечно-диастолического объема (КДО) и конечно-систолического объема (КСО) левого желудочка (ЛЖ) в I выборке: 104,4±4,2 мл и 45,8±3,5 мл соответственно, через год после АКШ – 113,3±5,6 мл и 52,4±4,7 мл (p<0,05). Подобная отрицательная динамика КДО и КСО ЛЖ во II группе не отмечалась. Качество жизни по Миннесотской шкале до АКШ в I когорте было 27,3±2,4 балла, а через 18 месяцев – 22,6±2,1 (р=0,126). Во II группе – 32,8±1,9, а на отдаленном этапе наблюдения – 20,9±2,2 балла (р<0,001). Острая декомпенсация хронической сердечной недостаточности (ОДХСН) в отдаленном периоде наблюдения в I группе развилась у 19 (32,2 %) больных, тогда как во II – у 3 (10,7 %) (ОШ 3,96; 95 % ДИ: 1,06–14,76; р=0,036). Заключение. У пациентов с вариантами Arg/Arg и Arg/Pro гена TP53 отмечается прогрессирование ремоделирования ЛЖ, а также в 4 раза повышенный риск развития ОДХСН в течение 18 месяцев после АКШ. Среди больных с аллелем Pro качество жизни после АКШ улучшается.

Introduction. Investigation for genetic determinants of cardiovascular diseases takes an important place in the concept of personalized medicine. Objective. To evaluate the effect of Arg72Pro (rs1042522) polymorphism TP53 gene on long-term prognosis in patients with coronary artery disease (CAD) undergoing coronary artery bypass grafting (CABG). Materials and methods. 89 patients with stable CAD and multivessel coronary artery disease (CA) were included in the study. The first sample was 61 people who were carriers of the Arg/Arg and Arg/Pro variants. The second one was 28 people who had the Pro/Pro variant. Patients were evaluated for myocardial remodeling, quality of life, as well as clinical data for the 18-months follow-up period. Results. In carriers of the Arg/Arg and Arg/Pro variants of the TP53 gene, during the follow-up period, we obtained elevation of end-diastolic volume (EDV) and end-systolic volume (ESV) of left ventricle (LV) in the first sample: 104.4 ± 4.2 ml and 45.8±3.5 ml, one year after CABG – 113.3±5.6 ml and 52.4±4.7 ml respectively (p<0.05). Negative dynamics of EDV and LV ESV in patients of group II were not observed. Quality of life by Minnesota scale before CABG in cohort I was 27.3±2.4 points, in 18 months after – 22.6±2.1 (p=0.126). In group II: 32.8±1.9, 1,5 years later – 20.9±2.2 points (p<0.001). Acute decompensation of chronic heart failure (ADHF) in the long-term follow-up period in group I occurred in 19 (32.2 %) patients, while in group II – in 3 persons (10.7 %) (OR 3.96; 95 % CI: 1.06-14.76; p=0.036). Conclusion. Patients with the Arg/Arg and Arg/Pro variants of the TP53 gene demonstrated progression of LV remodeling, as well as a 4-fold increased risk of ADHF within 18 months after CABG. In patients with the Pro allele, quality of life after CABG improved.

аортокоронарное шунтированиеишемическая болезнь сердцахроническая сердечная недостаточностьполиморфизм Arg72Pro гена ТP53полиморфизм rs1042522 гена ТP53ген апоптозаcoronary artery bypass graftingcoronary artery diseasechronic heart failureischemic heart diseaseArg72Pro polymorphism of TP53 genepolymorphism rs1042522 of TP53 geneapoptosis geneReferencesHeusch G. Myocardial ischemia: lack of coronary blood flow, myocardial oxygen supply-demand imbalance, or what? // Am J Physiol Heart Circ Physiol. 2019;316(6):H1439–H1446. Doi: 10.1152/ajpheart.00139.2019.Heusch G. Myocardial ischemia: lack of coronary blood flow, myocardial oxygen supply-demand imbalance, or what? // Am J Physiol Heart Circ Physiol. 2019;316(6):H1439–H1446. Doi: 10.1152/ajpheart.00139.2019.Галагудза М.М., Сонин Д.Л., Александров И.В. Гибернация миокарда: молекулярные механизмы, клиническая значимость и методы диагностики // Регионарное кровообращение и микроциркуляция. – 2019. – Т. 18, №3. – С. 9–15. Doi: 10.24884/1682-6655-2019-18-3-9-15.Galagudza MM, Sonin DL, Aleksandrov IV. Myocardial hibernation: molecular mechanisms, clinical significance and diagnostic methods // Regional blood circulation and microcirculation. 2019;18(3):9–15. (In Russ.). Doi: 10.24884/1682-6655-2019-18-3-9-15.Elsässer A, Vogt AM, Nef H, Kostin S, Möllmann H, Skwara W, Bode C, Hamm C, Schaper J. Human hibernating myocardium is jeopardized by apoptotic and autophagic cell death // J Am Coll Cardiol. 2004;43(12):2191–2199. Doi: 10. 1016/j.jacc.2004.02.053.Elsässer A, Vogt AM, Nef H, Kostin S, Möllmann H, Skwara W, Bode C, Hamm C, Schaper J. Human hibernating myocardium is jeopardized by apoptotic and autophagic cell death // J Am Coll Cardiol. 2004;43(12):2191–2199. Doi: 10. 1016/j.jacc.2004.02.053.Dumont P, Leu JI, Della Pietra AC 3rd, George DL, Murphy M. The codon 72 polymorphic variants of p53 have markedly different apoptotic potential // Nat Genet. 2003; 33(3):357–365. Doi: 10.1038/ng1093.Dumont P, Leu JI, Della Pietra AC 3rd, George DL, Murphy M. The codon 72 polymorphic variants of p53 have markedly different apoptotic potential // Nat Genet. 2003; 33(3):357–365. Doi: 10.1038/ng1093.Francisco G, Menezes PR, Eluf-Neto J, Chammas R. Arg72Pro TP53 polymorphism and cancer susceptibility: a comprehensive meta-analysis of 302 case-control studies // Int J Cancer. 2011;129(4):920–930. Doi: 10.1002/ijc.25710.Francisco G, Menezes PR, Eluf-Neto J, Chammas R. Arg72Pro TP53 polymorphism and cancer susceptibility: a comprehensive meta-analysis of 302 case-control studies // Int J Cancer. 2011;129(4):920–930. Doi: 10.1002/ijc.25710.Almeida A, Sánchez-Morán I, Rodríguez C. Mitochondrialnuclear p53 trafficking controls neuronal susceptibility in stroke // IUBMB Life. 2021;73(3):582–591. Doi: 10.1002/iub.2453.Almeida A, Sánchez-Morán I, Rodríguez C. Mitochondrialnuclear p53 trafficking controls neuronal susceptibility in stroke // IUBMB Life. 2021;73(3):582–591. Doi: 10.1002/iub.2453.Bonfigli AR, Sirolla C, Testa R, Cucchi M, Spazzafumo L, Salvioli S, Ceriello A, Olivieri F, Festa R, Procopio AD, Brandoni G, Boemi M, Marra M, Franceschi C. The p53 codon 72 (Arg72Pro) polymorphism is associated with the degree of insulin resistance in type 2 diabetic subjects: a cross-sectional study // Acta Diabetol. 2013;50(3):429–436. Doi: 10.1007/ s00592-012-0450-x.Bonfigli AR, Sirolla C, Testa R, Cucchi M, Spazzafumo L, Salvioli S, Ceriello A, Olivieri F, Festa R, Procopio AD, Brandoni G, Boemi M, Marra M, Franceschi C. The p53 codon 72 (Arg72Pro) polymorphism is associated with the degree of insulin resistance in type 2 diabetic subjects: a cross-sectional study // Acta Diabetol. 2013;50(3):429–436. Doi: 10.1007/ s00592-012-0450-x.Reiling E, Lyssenko V, Boer JM, Imholz S, Verschuren WM, Isomaa B, Tuomi T, Groop L, Dollé ME. Codon 72polymorphism (rs1042522) of TP53 is associated with changes in diastolic blood pressure over time // Eur J Hum Genet. 2012; 20(6):696–700. Doi: 10.1038/ejhg.2011.240.Reiling E, Lyssenko V, Boer JM, Imholz S, Verschuren WM, Isomaa B, Tuomi T, Groop L, Dollé ME. Codon 72polymorphism (rs1042522) of TP53 is associated with changes in diastolic blood pressure over time // Eur J Hum Genet. 2012; 20(6):696–700. Doi: 10.1038/ejhg.2011.240.Kolovou V, Tsipis A, Mihas C, Katsiki N, Vartela V, Koutelou M, Manolopoulou D, Leondiadis E, Iakovou I, Mavrogieni S, Kolovou G. Tumor Protein p53 (TP53) Gene and Left Main Coronary Artery Disease. Angiology // 2018;69(8):730– 735. Doi: 10.1177/0003319718760075.Kolovou V, Tsipis A, Mihas C, Katsiki N, Vartela V, Koutelou M, Manolopoulou D, Leondiadis E, Iakovou I, Mavrogieni S, Kolovou G. Tumor Protein p53 (TP53) Gene and Left Main Coronary Artery Disease. Angiology // 2018;69(8):730– 735. Doi: 10.1177/0003319718760075.Kulier A, Levin J, Moser R, Rumpold-Seitlinger G, Tudor IC, Snyder-Ramos SA, Moehnle P, Mangano DT; Investigators of the Multicenter Study of Perioperative Ischemia Research Group; Ischemia Research and Education Foundation. Impact of preoperative anemia on outcome in patients undergoing coronary artery bypass graft surgery // Circulation. 2007;116(5):471–479. Doi: 10.1161/CIRCULATIONAHA.106.653501.Kulier A, Levin J, Moser R, Rumpold-Seitlinger G, Tudor IC, Snyder-Ramos SA, Moehnle P, Mangano DT; Investigators of the Multicenter Study of Perioperative Ischemia Research Group; Ischemia Research and Education Foundation. Impact of preoperative anemia on outcome in patients undergoing coronary artery bypass graft surgery // Circulation. 2007;116(5):471–479. Doi: 10.1161/CIRCULATIONAHA.106.653501.Lee SI, Lee SY, Choi CH, Park CH, Park KY, Son KH. Relation between changes in red blood cell distribution width after coronary artery bypass grafting and early postoperative morbidity // J Thorac Dis. 2018;10(7):4244–4254. Doi: 10.21037/jtd.2018.06.108.Lee SI, Lee SY, Choi CH, Park CH, Park KY, Son KH. Relation between changes in red blood cell distribution width after coronary artery bypass grafting and early postoperative morbidity // J Thorac Dis. 2018;10(7):4244–4254. Doi: 10.21037/jtd.2018.06.108.Zindrou D, Taylor KM, Bagger JP. Preoperative haemoglobin concentration and mortality rate after coronary artery bypass surgery // Lancet. 2002;359(9319):1747–1748. Doi: 10.1016/S0140-6736(02)08614-2.Zindrou D, Taylor KM, Bagger JP. Preoperative haemoglobin concentration and mortality rate after coronary artery bypass surgery // Lancet. 2002;359(9319):1747–1748. Doi: 10.1016/S0140-6736(02)08614-2.Hannan EL, Samadashvili Z, Lahey SJ, Culliford AT, Higgins RS, Jordan D, Gold JP, Smith CR, Wechsler A. Predictors of postoperative hematocrit and association of hematocrit with adverse outcomes for coronary artery bypass graft surgery patients with cardiopulmonary bypass // J Card Surg. 2010; 25(6):638–646. Doi: 10.1111/j.1540-8191.2010.01143.x.Hannan EL, Samadashvili Z, Lahey SJ, Culliford AT, Higgins RS, Jordan D, Gold JP, Smith CR, Wechsler A. Predictors of postoperative hematocrit and association of hematocrit with adverse outcomes for coronary artery bypass graft surgery patients with cardiopulmonary bypass // J Card Surg. 2010; 25(6):638–646. Doi: 10.1111/j.1540-8191.2010.01143.x.Frentiu AA, Mao K, Caruana CB, Raveendran D, Perry LA, Penny-Dimri JC, Ramson DM, Segal R, Bellomo R, Smith JA, Liu Z. The Prognostic Significance of Red Cell Distribution Width in Cardiac Surgery: A Systematic Review and Meta-Analysis // J Cardiothorac Vasc Anesth. 2023;37(3):471– 479. Doi: 10.1053/j.jvca.2022.11.015.Frentiu AA, Mao K, Caruana CB, Raveendran D, Perry LA, Penny-Dimri JC, Ramson DM, Segal R, Bellomo R, Smith JA, Liu Z. The Prognostic Significance of Red Cell Distribution Width in Cardiac Surgery: A Systematic Review and Meta-Analysis // J Cardiothorac Vasc Anesth. 2023;37(3):471– 479. Doi: 10.1053/j.jvca.2022.11.015.Kertai MD, Zhou S, Karhausen JA, Cooter M, Jooste E, Li YJ, White WD, Aronson S, Podgoreanu MV, Gaca J, Welsby IJ, Levy JH, Stafford-Smith M, Mathew JP, Fontes ML. Platelet Counts, Acute Kidney Injury, and Mortality after Coronary Artery Bypass Grafting Surgery // Anesthesiology. 2016;124(2):339–352. Doi: 10.1097/ALN.0000000000000959.Kertai MD, Zhou S, Karhausen JA, Cooter M, Jooste E, Li YJ, White WD, Aronson S, Podgoreanu MV, Gaca J, Welsby IJ, Levy JH, Stafford-Smith M, Mathew JP, Fontes ML. Platelet Counts, Acute Kidney Injury, and Mortality after Coronary Artery Bypass Grafting Surgery // Anesthesiology. 2016;124(2):339–352. Doi: 10.1097/ALN.0000000000000959.Костенко В.А., Скородумова Е.А., Федоров А.Н., Кисельгоф О.Г. Влияние дисфункции почек с повышенным уровнем креатинина сыворотки крови на течение и прогноз инфаркта миокарда // Регионарное ровообращение и микроциркуляция. – 2013. – Т. 12, № 2. – С. 55–59. Doi: 10.24884/1682-6655- 2013-12-2-55-59.Kostenko VA, Skorodumova EA, Fedorov AN, Kiselgof OG. Influence of deteriorated renal function with elevated serum creatinin level for course of disease and prognosis of myocardinal infarction // Regional blood circulation and microcirculation. 2013;12(2):55–59. (In Russ.). Doi: 10.24884/1682-6655- 2013-12-2-55-59.Fallahzadeh A, Sheikhy A, Ajam A, Sadeghian S, Pashang M, Shirzad M, Bagheri J, Mansourian S, Momtahen S, Hosseini K. Significance of preoperative left ventricular ejection fraction in 5-year outcome after isolated CABG // J Cardiothorac Surg. 2021;16(1):353. Doi: 10.1186/s13019-021-01732-3.Fallahzadeh A, Sheikhy A, Ajam A, Sadeghian S, Pashang M, Shirzad M, Bagheri J, Mansourian S, Momtahen S, Hosseini K. Significance of preoperative left ventricular ejection fraction in 5-year outcome after isolated CABG // J Cardiothorac Surg. 2021;16(1):353. Doi: 10.1186/s13019-021-01732-3.Angelini A, Maiolino G, La Canna G, Ceconi C, Calabrese F, Pettenazzo E, Valente M, Alfieri O, Thiene G, Ferrari R. Relevance of apoptosis in influencing recovery of hibernating myocardium // Eur J Heart Fail. 2007;9(4):377–383. Doi: 10.1016/j.ejheart.2006.09.012.Angelini A, Maiolino G, La Canna G, Ceconi C, Calabrese F, Pettenazzo E, Valente M, Alfieri O, Thiene G, Ferrari R. Relevance of apoptosis in influencing recovery of hibernating myocardium // Eur J Heart Fail. 2007;9(4):377–383. Doi: 10.1016/j.ejheart.2006.09.012.Gloria-Bottini F, Saccucci P, Magrini A, Bottini E, Banci M, Papetti F, Nardi P, Scognamiglio M, Pellegrino A, Chiariello L. p53 codon 72 polymorphism and coronary artery disease: evidence of association with left ventricular ejection fraction // Am J Med Sci. 2012;343(2):127–130. Doi: 10.1097/ MAJ.0b013e318223ac71.Gloria-Bottini F, Saccucci P, Magrini A, Bottini E, Banci M, Papetti F, Nardi P, Scognamiglio M, Pellegrino A, Chiariello L. p53 codon 72 polymorphism and coronary artery disease: evidence of association with left ventricular ejection fraction // Am J Med Sci. 2012;343(2):127–130. Doi: 10.1097/ MAJ.0b013e318223ac71.Nagueh SF, Smiseth OA, Appleton CP, Byrd BF 3rd, Dokainish H, Edvardsen T, Flachskampf FA, Gillebert TC, Klein AL, Lancellotti P, Marino P, Oh JK, Popescu BA, Waggoner AD. Recommendations for the Evaluation of Left Ventricular Diastolic Function by Echocardiography: An Update from the American Society of Echocardiography and the European Association of Cardiovascular Imaging // J Am Soc Echocardiogr. 2016;29(4):277–314. Doi: 10.1016/j.echo.2016.01.011.Nagueh SF, Smiseth OA, Appleton CP, Byrd BF 3rd, Dokainish H, Edvardsen T, Flachskampf FA, Gillebert TC, Klein AL, Lancellotti P, Marino P, Oh JK, Popescu BA, Waggoner AD. Recommendations for the Evaluation of Left Ventricular Diastolic Function by Echocardiography: An Update from the American Society of Echocardiography and the European Association of Cardiovascular Imaging // J Am Soc Echocardiogr. 2016;29(4):277–314. Doi: 10.1016/j.echo.2016.01.011.Rector TS, Cohn JN. Assessment of patient outcome with the Minnesota Living with Heart Failure questionnaire: reliability and validity during a randomized, double-blind, placebo-controlled trial of pimobendan. Pimobendan Multicenter Research Group // Am Heart J. 1992;124(4):1017–1025. Doi: 10.1016/0002-8703(92)90986-6.Rector TS, Cohn JN. Assessment of patient outcome with the Minnesota Living with Heart Failure questionnaire: reliability and validity during a randomized, double-blind, placebo-controlled trial of pimobendan. Pimobendan Multicenter Research Group // Am Heart J. 1992;124(4):1017–1025. Doi: 10.1016/0002-8703(92)90986-6.Lagares MH, Silva KSF, Barbosa AM, Rodrigues DA, Costa IR, Martins JVM, Morais MP, Campedelli FL, Moura KKVO. Analysis of p53 gene polymorphism (codon 72) in symptomatic patients with atherosclerosis // Genet Mol Res. 2017;16(3). Doi: 10.4238/gmr16039721.Lagares MH, Silva KSF, Barbosa AM, Rodrigues DA, Costa IR, Martins JVM, Morais MP, Campedelli FL, Moura KKVO. Analysis of p53 gene polymorphism (codon 72) in symptomatic patients with atherosclerosis // Genet Mol Res. 2017;16(3). Doi: 10.4238/gmr16039721.Omrani-Nava V, Hedayatizadeh-Omran A, AlizadehNavaei R, Mokhberi V, Jalalian R, Janbabaei G, Amjadi O, Rahmatpour G, Mozaffari A. TP53 single nucleotide polymorphism (rs1042522) in Iranian patients with coronary artery disease // Biomed Rep. 2018;9(3):259–265. Doi: 10.3892/ br.2018.1121.Omrani-Nava V, Hedayatizadeh-Omran A, AlizadehNavaei R, Mokhberi V, Jalalian R, Janbabaei G, Amjadi O, Rahmatpour G, Mozaffari A. TP53 single nucleotide polymorphism (rs1042522) in Iranian patients with coronary artery disease // Biomed Rep. 2018;9(3):259–265. Doi: 10.3892/ br.2018.1121.Kojima S, Iwai N, Tago N, Ono K, Ohmi K, Tsujimoto G, Takagi S, Miyazaki S, Nonogi H, Goto Y. p53Arg72Pro polymorphism of tumour suppressor protein is associated with luminal narrowing after coronary stent placement // Heart. 2004;90(9):1069–1070. Doi: 10.1136/hrt.2002.007047.Kojima S, Iwai N, Tago N, Ono K, Ohmi K, Tsujimoto G, Takagi S, Miyazaki S, Nonogi H, Goto Y. p53Arg72Pro polymorphism of tumour suppressor protein is associated with luminal narrowing after coronary stent placement // Heart. 2004;90(9):1069–1070. Doi: 10.1136/hrt.2002.007047.Bojesen SE, Nordestgaard BG. The common germline Arg72Pro polymorphism of p53 and increased longevity in humans // Cell Cycle. 2008;7(2):158–63. Doi: 10.4161/cc.7.2.5249.Bojesen SE, Nordestgaard BG. The common germline Arg72Pro polymorphism of p53 and increased longevity in humans // Cell Cycle. 2008;7(2):158–63. Doi: 10.4161/cc.7.2.5249.

The authors declare that there are no conflicts of interest present.