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<article article-type="research-article" dtd-version="1.3" xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xml:lang="ru"><front><journal-meta><journal-id journal-id-type="publisher-id">microcirculation</journal-id><journal-title-group><journal-title xml:lang="ru">Регионарное кровообращение и микроциркуляция</journal-title><trans-title-group xml:lang="en"><trans-title>Regional blood circulation and microcirculation</trans-title></trans-title-group></journal-title-group><issn pub-type="ppub">1682-6655</issn><issn pub-type="epub">2712-9756</issn><publisher><publisher-name>Academician I.P. Pavlov First St. Petersburg State Medical University</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="doi">10.24884/1682-6655-2017-16-4-56-59</article-id><article-id custom-type="elpub" pub-id-type="custom">microcirculation-138</article-id><article-categories><subj-group subj-group-type="heading"><subject>Research Article</subject></subj-group><subj-group subj-group-type="section-heading" xml:lang="ru"><subject>ОРИГИНАЛЬНЫЕ СТАТЬИ (КЛИНИЧЕСКИЕ ИССЛЕДОВАНИЯ)</subject></subj-group><subj-group subj-group-type="section-heading" xml:lang="en"><subject>ORIGINAL ARTICLES (CLINICAL INVESTIGATIONS)</subject></subj-group></article-categories><title-group><article-title>Методика гемоперфузии изолированной почки крысы</article-title><trans-title-group xml:lang="en"><trans-title>A new protocol for isoheart rat kydney blood perfusion</trans-title></trans-title-group></title-group><contrib-group><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Минасян</surname><given-names>С. М.</given-names></name><name name-style="western" xml:lang="en"><surname>Minasian</surname><given-names>S. M.</given-names></name></name-alternatives><email xlink:type="simple">carkis@yandex.ru</email><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Мамедов</surname><given-names>А. Э.</given-names></name><name name-style="western" xml:lang="en"><surname>Mamedov</surname><given-names>A. E.</given-names></name></name-alternatives><email xlink:type="simple">ali-ma@mail.ru</email><xref ref-type="aff" rid="aff-2"/></contrib><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Полещенко</surname><given-names>Я. И.</given-names></name><name name-style="western" xml:lang="en"><surname>Poleshenko</surname><given-names>Ja. I.</given-names></name></name-alternatives><email xlink:type="simple">yana.poleschenko@gmail.com</email><xref ref-type="aff" rid="aff-2"/></contrib><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Скворцов</surname><given-names>А. Е.</given-names></name><name name-style="western" xml:lang="en"><surname>Scvortchov</surname><given-names>A. E.</given-names></name></name-alternatives><email xlink:type="simple">skvortsov.spb@gmail.com</email><xref ref-type="aff" rid="aff-3"/></contrib><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Резник</surname><given-names>О. Н.</given-names></name><name name-style="western" xml:lang="en"><surname>Reznik</surname><given-names>O. N.</given-names></name></name-alternatives><email xlink:type="simple">noemail@neicon.ru</email><xref ref-type="aff" rid="aff-3"/></contrib><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Кутенков</surname><given-names>А. А.</given-names></name><name name-style="western" xml:lang="en"><surname>Kutenkov</surname><given-names>A. A.</given-names></name></name-alternatives><email xlink:type="simple">noemail@neicon.ru</email><xref ref-type="aff" rid="aff-3"/></contrib><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Галагудза</surname><given-names>М. М.</given-names></name><name name-style="western" xml:lang="en"><surname>Galagudza</surname><given-names>M. M.</given-names></name></name-alternatives><email xlink:type="simple">galagoudza@mail.ru</email><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Сонин</surname><given-names>Д. Л.</given-names></name><name name-style="western" xml:lang="en"><surname>Sonin</surname><given-names>D. L.</given-names></name></name-alternatives><email xlink:type="simple">sonin_d@mail.ru</email><xref ref-type="aff" rid="aff-4"/></contrib><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Карпов</surname><given-names>А. А.</given-names></name><name name-style="western" xml:lang="en"><surname>Karpov</surname><given-names>A. A.</given-names></name></name-alternatives><email xlink:type="simple">a--karpoff@mail.ru</email><xref ref-type="aff" rid="aff-4"/></contrib><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Берко</surname><given-names>О. М.</given-names></name><name name-style="western" xml:lang="en"><surname>Berko</surname><given-names>O. M.</given-names></name></name-alternatives><email xlink:type="simple">noemail@neicon.ru</email><xref ref-type="aff" rid="aff-2"/></contrib><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Дмитриев</surname><given-names>Ю. В.</given-names></name><name name-style="western" xml:lang="en"><surname>Dmitriev</surname><given-names>Y. V.</given-names></name></name-alternatives><email xlink:type="simple">noemail@neicon.ru</email><xref ref-type="aff" rid="aff-4"/></contrib><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Власов</surname><given-names>Т. Д.</given-names></name><name name-style="western" xml:lang="en"><surname>Vlasov</surname><given-names>T. D.</given-names></name></name-alternatives><email xlink:type="simple">noemail@neicon.ru</email><xref ref-type="aff" rid="aff-1"/></contrib></contrib-group><aff-alternatives id="aff-1"><aff xml:lang="ru"><institution>Федеральное государственное бюджетное учреждение «Национальный медицинский исследовательский центр имени В. А. Алмазова» Министерства здравоохранения Российской Федерации; Федеральное государственное бюджетное образовательное учреждение высшего образования «Первый Санкт-Петербургский государственный медицинский университет им. акад. И. П. Павлова» Министерства здравоохранения Российской Федерации</institution><country>Россия</country></aff><aff xml:lang="en"><institution>The Almazov National Medical Research Centre; Pavlov First Saint Petersburg State Medical University</institution><country>Russian Federation</country></aff></aff-alternatives><aff-alternatives id="aff-2"><aff xml:lang="ru"><institution>Федеральное государственное бюджетное образовательное учреждение высшего образования «Первый Санкт-Петербургский государственный медицинский университет им. акад. И. П. Павлова» Министерства здравоохранения Российской Федерации</institution><country>Россия</country></aff><aff xml:lang="en"><institution>Pavlov First Saint Petersburg State Medical University</institution><country>Russian Federation</country></aff></aff-alternatives><aff-alternatives id="aff-3"><aff xml:lang="ru"><institution>Федеральное государственное бюджетное образовательное учреждение высшего образования «Первый Санкт-Петербургский государственный медицинский университет им. акад. И. П. Павлова» Министерства здравоохранения Российской Федерации; Государственное бюджетное учреждение «Санкт-Петербургский научно-исследовательский институт скорой помощи им. И. И. Джанелидзе» Министерства здравоохранения Российской Федерации</institution><country>Россия</country></aff><aff xml:lang="en"><institution>Pavlov First Saint Petersburg State Medical University; Institute of Emergency Medicine</institution><country>Russian Federation</country></aff></aff-alternatives><aff-alternatives id="aff-4"><aff xml:lang="ru"><institution>Федеральное государственное бюджетное учреждение «Национальный медицинский исследовательский центр имени В. А. Алмазова» Министерства здравоохранения Российской Федерации</institution><country>Россия</country></aff><aff xml:lang="en"><institution>The Almazov National Medical Research Centre</institution><country>Russian Federation</country></aff></aff-alternatives><pub-date pub-type="collection"><year>2017</year></pub-date><pub-date pub-type="epub"><day>30</day><month>12</month><year>2017</year></pub-date><volume>16</volume><issue>4</issue><fpage>56</fpage><lpage>59</lpage><permissions><copyright-statement>Copyright &amp;#x00A9; Минасян С.М., Мамедов А.Э., Полещенко Я.И., Скворцов А.Е., Резник О.Н., Кутенков А.А., Галагудза М.М., Сонин Д.Л., Карпов А.А., Берко О.М., Дмитриев Ю.В., Власов Т.Д., 2017</copyright-statement><copyright-year>2017</copyright-year><copyright-holder xml:lang="ru">Минасян С.М., Мамедов А.Э., Полещенко Я.И., Скворцов А.Е., Резник О.Н., Кутенков А.А., Галагудза М.М., Сонин Д.Л., Карпов А.А., Берко О.М., Дмитриев Ю.В., Власов Т.Д.</copyright-holder><copyright-holder xml:lang="en">Minasian S.M., Mamedov A.E., Poleshenko J.I., Scvortchov A.E., Reznik O.N., Kutenkov A.A., Galagudza M.M., Sonin D.L., Karpov A.A., Berko O.M., Dmitriev Y.V., Vlasov T.D.</copyright-holder><license xml:lang="ru" license-type="creative-commons-attribution" xlink:href="https://creativecommons.org/licenses/by/4.0/" xlink:type="simple"><license-p>Данная работа распространяется под лицензией Creative Commons Attribution 4.0.</license-p></license><license xml:lang="en" license-type="creative-commons-attribution" xlink:href="https://creativecommons.org/licenses/by/4.0/" xlink:type="simple"><license-p>This work is licensed under a Creative Commons Attribution 4.0 License.</license-p></license></permissions><self-uri xlink:href="https://www.microcirc.ru/jour/article/view/138">https://www.microcirc.ru/jour/article/view/138</self-uri><abstract><p>Введение и цель работы. Перфузия изолированных органов лабораторных животных является важным этапом оценки эффективности разрабатываемых способов консервации донорских органов. Материал и методы. Предложен способ гемоперфузии изолированной почки крысы после длительного периода гипотермической ишемии с целью воспроизведения реперфузии для последующей оценки морфологических изменений ткани почечной паренхимы. Результаты иследования. Данный способ оказался эффективным и технически более просто реализуемым, чем различные модификации классической методики трансплантации почки у мелких лабораторных животных. Выводы. Предлагаемая методика является альтернативой классической трансплантации почки в случае необходимости реперфузии в остром эксперименте в течение нескольких часов.</p></abstract><trans-abstract xml:lang="en"><p>Introduction and the purpose of the work. The perfusion of isolated laboratory animal organs is important for estimation of the effectiveness of methods elaborated for donor organ preservation. Materials and methods. Hemoperfusion of isolated rat kidney was performed after long-term hypothermal ischemia in order to reproduce reperfusion for the subsequent estimation of morphological changes in the cloth of nephritic parenchyma. Results. This method proved to be effective and technically simpler than different modifications of the classical procedure of kidney transplantation in small laboratory animals. Conclusions. The proposed procedure is an alternative to the classical kidney transplantation if reperfusion is required in an acute experiment for several hours.</p></trans-abstract><kwd-group xml:lang="ru"><kwd>экспериментальная перфузия почки</kwd><kwd>консервирующий раствор</kwd><kwd>experimental perfusion</kwd><kwd>preservation solution</kwd></kwd-group></article-meta></front><back><ref-list><title>References</title><ref id="cit1"><label>1</label><citation-alternatives><mixed-citation xml:lang="ru">Минасян С. М., Галагудза М. М., Дмитриев Ю. В. и др. 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