microcirculationРегионарное кровообращение и микроциркуляцияRegional blood circulation and microcirculation1682-66552712-9756Academician I.P. Pavlov First St. Petersburg State Medical University10.24884/1682-6655-2012-11-1-82-85microcirculation-482Research ArticleСтатьиИсследование нейропротективных эффектов теанина в различных дозах и на различных сроках введения при фокальной ишемии головного мозга у крыс.The neuroprotective effects of theanine in different doses and protocols of administration in the rat model of focal cerebral ischemiaДайнекоА. С.DaynekoA. S.noemail@neicon.ruСиманенковаА. В.SimanenkovaA. V.noemail@neicon.ruПросвирнинаМ. С.ProsvirninaM. S.noemail@neicon.ruЗухуроваМ. АZuhurovaM. A.noemail@neicon.ruВласовТ. Д.VlasovT. D.tvlasov@yandex.ruСанкт-Петербургский государственный медицинский университет им. акад. И. П. ПавловаРоссияSaint-Petersburg Pavlov State Medical UniversityRussian Federation2012300320121118285Copyright © Дайнеко А.С., Симаненкова А.В., Просвирнина М.С., Зухурова М.А., Власов Т.Д., 20122012Дайнеко А.С., Симаненкова А.В., Просвирнина М.С., Зухурова М.А., Власов Т.Д.Dayneko A.S., Simanenkova A.V., Prosvirnina M.S., Zuhurova M.A., Vlasov T.D.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.microcirc.ru/jour/article/view/482

Рассматривается нейропротективный эффект гамма-глутамилэтиламида (L-теанина) при ишемическом повреждении головного мозга крыс, вызванном окклюзией левой средней мозговой артерии (ЛСМА). Теанин вводился внутрибрюшинно в дозах 1 мг/кг и 4 мг/кг за 30 минут до и через 3, 12 и 24 часа после окклюзии ЛСМА. Теанин, введенный за 30 минут до ишемии и через 3 и 12 часов после начала реперфузии, достоверно уменьшал размеры зоны некроза на 2-е сутки после ишемии. Кроме того, при оценке состояния животных на 2-й день после воспроизведения окклюзии ЛСМА отмечалось достоверное уменьшение неврологического дефицита у крыс тех же экспериментальных групп. Теанин, введенный через 24 часа после начала реперфузии, достоверно не изменял размер зоны некроза и степень выраженности неврологического дефицита. Таким образом, доказано, что применение теанина до ишемии и через 3 и 12 часов после нее обеспечивает защиту головного мозга, что потенциально может быть использовано для профилактики и терапии сосудистых заболеваний головного мозга.

This study was aimed to evaluate neuroprotective effect of gamma-glutamylethylamide (L-theanine) in brain ischemic damage in rats, caused by left middle cerebral artery occlusion. Theanine was administered intraperitoneally 1mg/kg and 4 mg/kg 30 minutes before ischemia and 3, 12, 24 hours after left middle cerebral artery occlusion. The necrosis size in rats that received theanine 30 minutes before ischemia and 3 and 12 hours after the beginning of reperfusion was significantly decreased 2 days after ischemia. Furthermore, the assessment of animal status on the second day after left middle cerebral artery occlusion revealed significant neurological deficiency reduction in the same experimental groups. Theanine, administered 24 hours after the beginning of reperfusion, failed to change necrosis size and neurological deficiency extent significantly. Thereby, it has been proved, that theanine, injected before brain ischemia and 3 and 12 hours after it, provides brain protection, that potentially might be used in prophylactic and therapy of brain vascular diseases.

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The authors declare that there are no conflicts of interest present.