microcirculationРегионарное кровообращение и микроциркуляцияRegional blood circulation and microcirculation1682-66552712-9756Academician I.P. Pavlov First St. Petersburg State Medical University10.24884/1682-6655-2012-11-3-69-75microcirculation-900Research ArticleЭКСПЕРИМЕНТАЛЬНЫЕ ИССЛЕДОВАНИЯEXPERIMENTAL INVESTIGATIONSПерспективы использования микроРНК в качестве биомаркера ишемического повреждения миокардаMicroRNA as biomarkers of myocardial ischemic injury: a perspectiveФедоровА. В.FedorovA. V.noemail@neicon.ruКостареваА. А.KostarevaA. A.noemail@neicon.ruГалагудзаМ. М.GalagudzaM. M.galagoudza@mail.ruМинасянС. М.MinasyanS. M.noemail@neicon.ruКурапеевД. И.KurapeevD. I.noemail@neicon.ruФедеральный центр сердца, крови и эндокринологии им. В. А. АлмазоваРоссияV. A. Almazov Federal Center of Heart, Blood and EndocrinologyRussian FederationФедеральный центр сердца, крови и эндокринологии им. В. А. Алмазова; Санкт-Петербургский государственный медицинский университет им. акад. И. П. ПавловаРоссияV. A. Almazov Federal Center of Heart, Blood and Endocrinology; I. P. Pavlov State Medical UniversityRussian Federation2012300920121136975Copyright © Федоров А.В., Костарева А.А., Галагудза М.М., Минасян С.М., Курапеев Д.И., 20122012Федоров А.В., Костарева А.А., Галагудза М.М., Минасян С.М., Курапеев Д.И.Fedorov A.V., Kostareva A.A., Galagudza M.M., Minasyan S.M., Kurapeev D.I.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.microcirc.ru/jour/article/view/900

В последние годы важная роль микроРНК в регуляции функции сердечно-сосудистой системы становится общепризнанной. Усовершенствование методов выделения и анализа экспрессии микроРНК позволило выявить эти молекулы не только в клетках, но и в различных биологических жидкостях. Несмотря на то, что механизмы выхода микроРНК в биологические жидкости, а также функции внеклеточной микроРНК во многом остаются не известными, появляется все больше работ об использовании циркулирующих микроРНК в качестве биомаркеров различных патологических процессов. В настоящем обзоре обсуждаются методы количественного определения циркулирующих микроРНК и приводятся данные о повышении уровней определенных микроРНК в крови в ответ на ишемическое повреждение миокарда.

Over the past few years, it has become evident that microRNA is involved in the regulation of cardiovascular function. The continuous refinement of the techniques of microRNA purification and analysis has allowed the identification of these molecules not only in the cells, but also in the different biological fluids. Despite the fact that the mechanisms of microRNA release into the biological fluids as well as the functions of extracellular microRNA remain largely unexplored, the number of publications on the use of circulating microRNAs as biomarkers of various pathological processes is increasing. In this review, we discuss the methods of quantitative determination of circulating microRNA and summarize recent data on increased levels of certain microRNAs in the blood in response to myocardial ischemia.

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The authors declare that there are no conflicts of interest present.