Distinction of experimental wounds microcirculation under local immunotherapy
https://doi.org/10.24884/1682-6655-2022-21-1-53-58
Abstract
Introduction. Changes in local microcirculation are essential in the treatment of infectious surgical diseases. Aim of the study to evaluate the features of microcirculation while comparing local treatment with aqueous dispersions of oxide nanostructures of metals, myelopide and chlorhexidine bigluconate in experimental conditions on animals.
Materials and methods. Experimental modeling was performed on white Wistar rats of both sexes. 6 groups were formed: 1 – withdrawn from the experiment on the 1st day; 2 – control group without treatment; 3 – treatment with an aqueous dispersion of metal oxide nanostructures (group 1); 4 – treatment with a solution of 300 µg of myelopide per 1 ml (group 2); 5 – treatment with a solution of 600 μg of myelopide per 1 ml (group 3); 6. treatment with an aqueous solution of chlorhexidine bigluconate 0.05 % (group 4). The study was carried out on the 1st and 7th days of treatment, determining the number of capillaries per 0.1 mm2 of biopsy specimens. The data were processed with the determination of the mean values (M±m), 1st and 3rd quartiles [Q1-Q3], standard deviation (σ), correlation coefficient (CV, %), coefficient of Student’s.
Results. On the first day, the animals had an average of 2.5±0.9 capillaries per 0.1 mm2 of wound area. On the 7th day, the lowest vascularization was in the control group 2.7±0.9 per 0.1 mm2 . The largest number of vessels was determined in the 1st observation group using aqueous dispersions of metal oxide nanostructures. The correlation coefficients were 40–56 %. On the 7th day, the values of neoangiogenesis during local immunocorrection using an aqueous solution of myelopide were similar.
Conclusions. The least vascularization was noted in the control group (2.7±0.9). The greatest changes were associated with the use of aqueous dispersions of metal oxide nanostructures (3.8±1.4). The differences in the healing dynamic were associated with neoangiogenesis and the multifactorial nature of local immunocorrection.
About the Authors
A. S. Moshkin
Orel State University named after I. S. Turgenev
Russian Federation
Russian Federation
Moshkin Andrey S. – PhD., Associate Professor of the Department of Anatomy, Operative Surgery and Disaster Medicine
95, Komsomolskaya str., Orel, Russia, 302026
M. A. Khalilov
Orel State University named after I. S. Turgenev
Russian Federation
Russian Federation
Khalilov Maksud A. – MD, PhD., Professor, Head of the Department of Anatomy, Operative Surgery and Disaster Medicine
95, Komsomolskaya str., Orel, Russia, 302026
A. B. Bochkarev
Orel State University named after I. S. Turgenev
Russian Federation
Russian Federation
Bochkarev Alexey B. – PhD., Associate Professor of the Department of Anatomy, Operative Surgery and Disaster Medicine
95, Komsomolskaya str., Orel, Russia, 302026
References
1. Khalilov MA, Snimchsikova IA. Experience in local immunocorrectionin treatment of chronic wounds // Medical immunology. 2010;12(3):227–234. (In Russ).
2. Duvansky VA, Ovsyannikov VS, Biryukov AYu. Evaluation of microcirculation of purulent wounds // Laser medicine. 2016; 20(3):102. (In Russ.)].
3. Fedyanin SD. Method of stimulation of wound healing in patients with surgical infection // Surgery. Eastern Europe. 2020;9(4):351–356. (In Russ.).
4. Lipatov KV, Cherkasov YuE, Khrupkin VI, Lysenko MV, Dekheisi EI. Differentiated tactics in the complex surgical treatment of carbuncles // Military Medical Journal. 2020;341(6):28–32. (In Russ.).
5. Zeimakh EA, Zinchenko VYu. The importance of eliminating microcirculatory disorders in the complex treatment of common soft tissue phlegmon // Acta Biomedica Scientifica (East Siberian Biomedical Journal). 2021;6(5):212–221. (In Russ.). Doi 10.29413/ABS. 2021-6.5.20.
6. Grigoryan AYu, Belozerova AV. The use of some antiseptics in the treatment of experimental purulent wounds // Youth innovation Bulletin. 2017;6(1):23–25. (In Russ.).
7. Andreev VA, Sboychakov VB, Narolskaya DP, Semenova DK. New approaches to the treatment of purulent wounds // V Luga scientific readings. Modern scientific knowledge: theory and practice materials of the international scientific conference. 2017;170–173. (In Russ.).
8. Glukhov AA, Alekseeva NT, Ostroushko AP. Morphofunctional changes in tissues during wound healing against the background of the use of platelet concentrate // News of surgery. 2013;21(1):12–22. (In Russ.).
9. Grigoriev AG, Grigorieva AA, Orlinskaya NYu, Shevantaeva ON, Shchelchkova NA, Mironov AA. Morphological features of healing of purulent wounds when using cryoxygen method // Morphological Vedomosti. 2015; (1):37–42. (In Russ.).
10. Fomin AA, Mainugin SV, Krasavin VA, Kazmiruk NA, Khudoyarov TA, Kamensky SYu. Laser Doppler imaging in the practice of purulent surgery department // Thrombosis, hemostasis and rheology. 2012; 4(52):50–56. (In Russ.).
Review
For citations:
Moshkin A.S., Khalilov M.A., Bochkarev A.B. Distinction of experimental wounds microcirculation under local immunotherapy. Regional blood circulation and microcirculation. 2022;21(1):53-58. (In Russ.) https://doi.org/10.24884/1682-6655-2022-21-1-53-58
Views:
507
Email this article 
