Influence of polylactide wound dressings with tannic acid on serum concentration of markers of angiogenesis, endothelial cell alteration and activation in white rats during healing of experimental full-thickness skin defect
A. N. Ivanov,
M. A. Sahan,
A. V. Ermakov,
A. A. Savkina,
V. V. Nikitina,
T. V. Stepanova,
E. V. Lengert,
T. S. Kiriyazi https://doi.org/10.24884/1682-6655-2024-23-3-81-88
Abstract
Introduction. The development of effective treatment of skin wounds is the one of the current challenges. Synthetic wound dressings based on polylactide, capable of targeted delivery of biologically active substances, provide new solutions to the problem of stimulating regeneration. Tannic acid seems to be a promising substance as a component of wound dressings due to its antiinflammatory and antioxidant effects. The aim of the study was to assess the changes in the serum concentrations of markers of angiogenesis, endothelial cell alteration and activation under the influence of microchamber polylactide wound dressings loaded with tannic acid during the healing of an experimental full-thickness skin defect in white rats. Materials and Methods. The experiment was carried out on 63 white male rats, divided into four groups: intact animals (n=9), comparison group (n=18), experimental groups No. 1, 2 (n=18 each). A 10×10 mm acute excisional skin wound model was surgically created in animals of the comparative and experimental groups. Animals in the experimental group № 1 received a polylactide microchamber wound dressing without active components on the formed skin defect. The rats of experimental group № 2 received a similar dressing with its microchambers loaded with tannic acid. The concentrations of vascular endothelial growth factor (VEGF), syndecan-1, and sE-selectin in the blood were assessed. Results. It was found that the healing of a full-thickness skin defect in rats is accompanied by alteration and activation of the endothelium, characterized by an increase in serum concentrations of syndecan-1 in 4.4 times and sE-selectin in 2.2 times. There is also an activation of angiogenesis with increase in VEGF concentration 1.7 times on the 7th day and 6.5 times on the 14th day of the experiment. Dressings with tannic acid cause a decrease in the alteration of the endothelial glycocalyx and inflammatory activation of endothelial cells, which is manifested by a decrease in serum concentrations of VEGF and syndecan-1 by 2 times and sE-selectin by 1.7 times on the 7th day of the experiment compared to the analogue without active components and complete normalization of the serum concentration of the angiogenesis stimulator VEGF by the 14th day of the experiment. Conclusion. Polylactide wound dressings with micro-chambers filled with tannic acid have a significant effect on the course of the wound process in rats with a full-thickness skin defect.
Keywords
wound dressings,
wound defect of the skin,
tannic acid,
polylactic acid,
vascular endothelial growth factor,
syndecan-1,
sE-selectin
Supporting agencies: The study was supported by the state assignment of the Razumovsky Saratov State Medical University of the Ministry of Health of Russia «Development of microchamber wound dressings providing local modulation of tissue oxidative homeostasis».
About the Authors
A. N. Ivanov
V. I. Razumovsky Saratov State Medical University
Russian Federation
Russian Federation
Ivanov Alexey N. – Doctor of Medical Sciences, Head, Department of Laboratory Diagnostics, Research Institute of Traumatology, Orthopedics and Neurosurgery; Head, Central Scientific Research Laboratory; Head, Department of Normal Physiology named after I. A. Chuevsky,
112, Bolshaya Kazachya str., Saratov, 410012.
M. A. Sahan
V. I. Razumovsky Saratov State Medical University
Russian Federation
Russian Federation
Sahan Maksim A. – Assistant, Department of Normal Physiology named after I.A. Chuevsky; Junior Researcher, Central Research Laboratory,
112, Bolshaya Kazachya str., Saratov, 410012.
A. V. Ermakov
V. I. Razumovsky Saratov State Medical University
Russian Federation
Russian Federation
Ermakov Alexey V. – Candidate (PhD) of Physical and Mathematical Sciences, Senior Researcher, Central Research Laboratory,
112, Bolshaya Kazachya str., Saratov, 410012.
A. A. Savkina
V. I. Razumovsky Saratov State Medical University
Russian Federation
Russian Federation
Savkina Angelina A. – Junior Researcher, Central Research Laboratory,
112, Bolshaya Kazachya str., Saratov, 410012.
V. V. Nikitina
V. I. Razumovsky Saratov State Medical University
Russian Federation
Russian Federation
Nikitina Victoria V. – Candidate (PhD) of Medical Sciences, Associate Professor, Department of Biochemistry, Senior Researcher, Central Research Laboratory,
112, Bolshaya Kazachya str., Saratov, 410012.
T. V. Stepanova
V. I. Razumovsky Saratov State Medical University
Russian Federation
Russian Federation
Stepanova Tatyana V. – Junior Researcher, Central Research Laboratory,
112, Bolshaya Kazachya str., Saratov, 410012.
E. V. Lengert
V. I. Razumovsky Saratov State Medical University
Russian Federation
Russian Federation
Lengert Ekaterina V. – Junior Researcher, Central Research Laboratory,
112, Bolshaya Kazachya str., Saratov, 410012.
T. S. Kiriyazi
V. I. Razumovsky Saratov State Medical University
Russian Federation
Russian Federation
Kiriyazi Tatyana S. – Candidate (PhD) of Biological Sciences, Associate Professor, Department of Normal Physiology named after I. A. Chuevsky, Senior Researcher, Central Research Laboratory,
112, Bolshaya Kazachya str., Saratov, 410012.
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Review
For citations:
Ivanov A.N., Sahan M.A., Ermakov A.V., Savkina A.A., Nikitina V.V., Stepanova T.V., Lengert E.V., Kiriyazi T.S. Influence of polylactide wound dressings with tannic acid on serum concentration of markers of angiogenesis, endothelial cell alteration and activation in white rats during healing of experimental full-thickness skin defect. Regional blood circulation and microcirculation. 2024;23(3):81-88. (In Russ.) https://doi.org/10.24884/1682-6655-2024-23-3-81-88
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