Experimental Model of Superficial Focal Ischemic Cerebral Infarction in Rats
https://doi.org/10.24884/1682-6655-2026-25-1-85-92
Abstract
Introduction. Experimental models of cerebral ischemia are widely used in preclinical studies, but they are often insufficiently adapted for studying thrombosis and thrombolysis in the microcirculation.
Objective. To develop a new approach for modeling focal infarction of the cerebral cortex in the parietal zone in rats.
Materials and Methods. The approach is based on the application of a cotton thread soaked in a 24.4% iron (III) chloride to the dura mater at the location of the pial arteriole.
Results. A 10-minute application was found to cause narrowing of the arteriole lumen and dilation of nearby venules, as well as a significant decrease in cerebral blood flow. After 24 hours, an infarction zone of approximately 22 mm2 was formed in this area. Administration of tissue plasminogen activator (2 mg/kg, intra-arterially) at the beginning of the application suppressed occlusion, eliminated the decrease in cerebral blood flow (27.5 vs 3.3 PU, p<0.001), and significantly decreased the area of cerebral cortex damage (0.33 vs 22 mm2, p<0.0001).
Conclusion. The developed method allows for intravital recording of microcirculation changes and it can be used to explore new approaches to thrombolytic therapy for stroke.
Supporting agencies: The work was carried out within the framework of the state assignment № FGFU-2025-0004 «Molecular-cellular and systemic mechanisms of brain dysfunction in neurodegenerative diseases and its aging: development of diagnostic, treatment and prevention technologies»
About the Authors
V. V. Aleksandrin
Institute of General Pathology and Pathophysiology
Russian Federation
Russian Federation
Aleksandrin Valeriy V. – Candidate (PhD) of Biological Sciences, Senior Researcher, Laboratory of Blood Aggregation Regulation.
8, Baltiyskaya str., Moscow, 125315
A. V. Ivanov
Institute of General Pathology and Pathophysiology
Russian Federation
Russian Federation
Ivanov Alexander V. – Candidate (PhD) of Medical Sciences, Senior Researcher, Laboratory of Blood Aggregation Regulation.
8, Baltiyskaya str., Moscow, 125315
M. A. Popov
Institute of General Pathology and Pathophysiology
Russian Federation
Russian Federation
Popov Mikhail A. – Candidate (PhD) of Medical Sciences, Senior Researcher, Laboratory of Angiopathology.
8, Baltiyskaya str., Moscow, 125315
A. G. Filippov
Institute of General Pathology and Pathophysiology
Russian Federation
Russian Federation
Filippov Alexander G. – Junior Researcher, Laboratory of Cell Biology and Developmental Pathology.
8, Baltiyskaya str., Moscow, 125315
A. A. Kubatiev
Institute of General Pathology and Pathophysiology
Russian Federation
Russian Federation
Kubatiev Aslan A. – Member of the Russian Academy of Sciences, Professor, MD, Scientific Director.
8, Baltiyskaya str., Moscow, 125315
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Review
For citations:
Aleksandrin V.V., Ivanov A.V., Popov M.A., Filippov A.G., Kubatiev A.A. Experimental Model of Superficial Focal Ischemic Cerebral Infarction in Rats. Regional blood circulation and microcirculation. 2026;25(1):85-92. (In Russ.) https://doi.org/10.24884/1682-6655-2026-25-1-85-92
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