Comparative evaluation of mesenchymal stem cells cultivation methods on biodegradable L-polylactide scaffold for the creation of tissue-engineered vascular implant
https://doi.org/10.24884/1682-6655-2018-17-1-61-68
Abstract
Cell integration and cultivation on the matrix are the key processes in the development of a tissue-engineered vascular implant (TEVI) based on a biodegradable polymer scaffold. The aim of this researh is to identify the optimal method of mesenchymal stem cells cultivation on the tubular biodegradable scaffold from L-polylactide. The comparative evaluation of the static and dynamic cultivation methods was performed. Proposed method of combining filtration method of cell integration and dynamic cultivation has proved its higher efficiency and is suitable for further development of TEVI.
About the Authors
G. I. Popov
Pavlov First Saint Petersburg State Medical University
Russian Federation
Russian Federation
A. E. Kryukov
Institute of Macromolecular Compounds, Russian Academy of Sciences; Peter the Great Saint-Petersburg State Polytechnical University
Russian Federation
Russian Federation
Yu. A. Nashchekina
Peter the Great Saint-Petersburg State Polytechnical University; Institute of Cytology of the Russian Academy of Science
Russian Federation
Russian Federation
E. M. Ivankova
Institute of Macromolecular Compounds, Russian Academy of Sciences; Peter the Great Saint-Petersburg State Polytechnical University
Russian Federation
Russian Federation
V. N. Vavilov
Pavlov First Saint Petersburg State Medical University
Russian Federation
Russian Federation
V. E. Yudin
Institute of Macromolecular Compounds, Russian Academy of Sciences; Peter the Great Saint-Petersburg State Polytechnical University
Russian Federation
Russian Federation
P. V. Popryadukhin
Institute of Macromolecular Compounds, Russian Academy of Sciences; Peter the Great Saint-Petersburg State Polytechnical University
Russian Federation
Russian Federation
G. Yu. Yukina
Pavlov First Saint Petersburg State Medical University
Russian Federation
Russian Federation
N. V. Smirnova
Institute of Macromolecular Compounds, Russian Academy of Sciences; Peter the Great Saint-Petersburg State Polytechnical University
Russian Federation
Russian Federation
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Review
For citations:
Popov G.I., Kryukov A.E., Nashchekina Yu.A., Ivankova E.M., Vavilov V.N., Yudin V.E., Popryadukhin P.V., Yukina G.Yu., Smirnova N.V. Comparative evaluation of mesenchymal stem cells cultivation methods on biodegradable L-polylactide scaffold for the creation of tissue-engineered vascular implant. Regional blood circulation and microcirculation. 2018;17(1):61-68. (In Russ.) https://doi.org/10.24884/1682-6655-2018-17-1-61-68
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