Rat’s behaviour in eight-arms maze after modeling of focal transient cerebral ischemia with subsequent activation of striatal microglia
https://doi.org/10.24884/1682-6655-2018-17-2-92-96
Abstract
It is well known that cognitive disorders at cerebral ischemia-reperfusion are followed by neuronal death. The inflammation of nervous tissue occurs, but the role of microgliocytes in neuroprotection is poorly understood. The aim of our work was to study microgliocytes in rats with cognitive disorders in delayed postischemic period after focal transient cerebral ischemia. In an experiment we used male rats (n=15), weighing 200-250. The model of focal brain ischemia was performed in anaesthetized rats (intraperitonealy, chloralhydrate 450 mg/kg). Microsurgical introduction monofilament to the left middle cerebral artery was made by J. Koizumi technique. Time of middle cerebral artery (MCA) occlusion was 30 minutes with the subsequent reperfusion period. Testing in eight-arm maze was performed within seven days twice with a two-day interval during which each animal carried out a 5-minute session 3 times with a 3-minute interval. For identification of microgliocytes used polyclonal goat antibodies to Iba1 antigen. At behavior assessment in an eight-arm maze there were errors of working memory revealed and prolongation of time of task performance. Cognitive disorders at cerebral ischemia, perhaps, serve as the neuroinflammation indicator. Microglia is likely involved in mechanisms of neuroprotection and compensation for cognitive disorders.
Keywords
ишемия,
восьмирукавный лабиринт,
микроглия,
головной мозг,
ischemia,
eight-arm maze,
microglia,
brain
About the Authors
M. E. Kolpakova
Federal State Budgetary Educational Institution of Higher Education «Pavlov First Saint Petersburg State Medical University»
Russian Federation
Russian Federation
I. A. Filchenko
Federal State Budgetary Educational Institution of Higher Education «Pavlov First Saint Petersburg State Medical University»
Russian Federation
Russian Federation
A. . Trajkovski
Federal State Budgetary Educational Institution of Higher Education «Pavlov First Saint Petersburg State Medical University»
Russian Federation
Russian Federation
D. L. Tcyba
Federal State Budgetary Educational Institution of Higher Education «Pavlov First Saint Petersburg State Medical University»
Russian Federation
Russian Federation
O. V. Kirik
Federal State Budgetary Scientific Institution «Institute of Experimental Medicine»
Russian Federation
Russian Federation
Arieb A. Khan
Federal State Budgetary Educational Institution of Higher Education «Pavlov First Saint Petersburg State Medical University»
Russian Federation
Russian Federation
A. . Leipert
Federal State Budgetary Educational Institution of Higher Education «Pavlov First Saint Petersburg State Medical University»
Russian Federation
Russian Federation
D. E. Korzhevskii
Federal State Budgetary Scientific Institution «Institute of Experimental Medicine»
Russian Federation
Russian Federation
T. D. Vlasov
Federal State Budgetary Educational Institution of Higher Education «Pavlov First Saint Petersburg State Medical University»
Russian Federation
Russian Federation
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Review
For citations:
Kolpakova M.E., Filchenko I.A., Trajkovski A., Tcyba D.L., Kirik O.V., Khan A.A., Leipert A., Korzhevskii D.E., Vlasov T.D. Rat’s behaviour in eight-arms maze after modeling of focal transient cerebral ischemia with subsequent activation of striatal microglia. Regional blood circulation and microcirculation. 2018;17(2):92-96. (In Russ.) https://doi.org/10.24884/1682-6655-2018-17-2-92-96
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