The investigation of infarct-limiting effects of preservation solutions in the rat model of long-time static cold storage of isolated rat heart

Abstract

One of the most important aims of modern transplantation is the preservation of the morphofunctional state of donor organs for as long as possible time after removing them from the donor prior to implantation into a patient. To solve this problem, heart preservation solutions are used. However, despite the large number of prescriptions of preservation solutions, the time, limited to six hours, after removing donor heart is still a limiting factor in determining the possibility of implanting heart into a patient. In this study, cardioprotective effects of widely used in clinical practice preservation solution HTK and new Krebs-Henseleit buffer-based preservation solution were studied in the rat model of prolonged static cold storage. It was shown that new Krebs-Henseleit buffer-based preservation solution protected the heart significantly even after an eight-hour global ischemia and has the advantage over Custodiol in the infarct-limiting action.