Simulation of photodynamically-induced thrombosis of the femoral vein of rats
https://doi.org/10.24884/1682-6655-2019-18-2-64-70
Abstract
The purpose of the work is to determine the conditions that ensure the formation of a stable thrombus in the femoral vein of rats with photodynamic damage.
Material and methods. The experiments were carried out on male Wistar rats weighing 250–270 g. A photosensitizer (FS) Bengal rose (ACROS ORGANICS, USA) (BR) was administered intravenously at a dose of 17 mg/kg and after 60 minutes a vascular clamp was applied to the femoral vein. Distal to clamping, a 30-minute laser irradiation of a vein was performed (DPSS diode laser, Republic of South Korea, 532 nm, 60 mW, irradiation area – 1 mm2 ). The clamp was removed after 90 min after cessation of irradiation. The blood flow was recorded by the method of high-frequency Doppler ultrasound (MinimaxDoppler-K Russia). Cessation of blood flow was considered as an indicator of occlusion of femoral vein.
Results. Rats, after the clamp was removed, had no blood flow in the irradiated veins, and after 24 hours it was not restored. Histology revealed severe damage of all layers of the vascular wall and the formation of a blood clot filling the lumen of the vessel. After a 30-minute irradiation without clamping the vein, the blood flow was maintained, although damage to the vascular wall was detected. Introduction of BR and clipping of the vessel without irradiation caused minimal damage to the vascular wall and did not lead to thrombosis.
About the Authors
E. Y. Vasina
Pavlov University
Russian Federation
Vasina Elena Yu. – Candidate of Medical Sciences, Associate Professor of the Department of Pathophysiology with a course of clinical pathophysiology
Russian Federation
Vasina Elena Yu. – Candidate of Medical Sciences, Associate Professor of the Department of Pathophysiology with a course of clinical pathophysiology
E. G. Sukhorukova
Pavlov University
Russian Federation
Sukhorukova Elena G. – Candidate of Medical Sciences, Senior Research Associate of the Laboratory of Pathomorphology of the Research Center
Russian Federation
Sukhorukova Elena G. – Candidate of Medical Sciences, Senior Research Associate of the Laboratory of Pathomorphology of the Research Center
S. G. Chefu
Pavlov University; Almazov National Medical Research Centre
Russian Federation
Chefu Svetlana G. – Candidate of Biological Sciences, Head of Experimental Research Laboratory of the Laser Medicine Center
Russian Federation
Chefu Svetlana G. – Candidate of Biological Sciences, Head of Experimental Research Laboratory of the Laser Medicine Center
G. Yu. Yukina
Pavlov University
Russian Federation
Yukina Galina Yu. – Candidate of Biological Sciences, Head of the Laboratory of Pathomorphology of the Research Center
Russian Federation
Yukina Galina Yu. – Candidate of Biological Sciences, Head of the Laboratory of Pathomorphology of the Research Center
N. N. Petrishchev
Pavlov University; Almazov National Medical Research Centre
Russian Federation
Petrishchev Nikolay N. – Dr. habil. of Medical Sciences, Professor of the Department of Pathophysiology with a course of clinical pathophysiology
Russian Federation
Petrishchev Nikolay N. – Dr. habil. of Medical Sciences, Professor of the Department of Pathophysiology with a course of clinical pathophysiology
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Review
For citations:
Vasina E.Y., Sukhorukova E.G., Chefu S.G., Yukina G.Yu., Petrishchev N.N. Simulation of photodynamically-induced thrombosis of the femoral vein of rats. Regional blood circulation and microcirculation. 2019;18(2):64-69. (In Russ.) https://doi.org/10.24884/1682-6655-2019-18-2-64-70
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