Structural and functional peculiarities of the endothelium of heart vessels of mature rats according to immunistochemical studies
https://doi.org/10.24884/1682-6655-2019-18-2-70-77
Abstract
The relevance of the study is connected with the modern point of view that endothelial dysfunction may be the cause of such socially significant diseases as atherosclerosis, diabetes, varicose veins, etc. The aim of the work is to study the morphofunctional peculiarities of the endothelium of the cardiovascular vessels of the mature rat using Willebrand factor (vWF) immunohistochemistry.
Material and methods. We used mature Wistar rats (n = 12). Polyclonal rabbit antibodies were used for the immunohistochemical detection of vWF. The reaction was carried out on paraffin sections, made through the whole heart.
Results. It was shown that the immunopositive reaction to vWF (vWF+ ) in the endothelium of different regions of the rat heart is not uniform. A tendency was found to weaken the vWF+ reaction in the direction from the base of the heart to its apex. Most functionally active endothelial cells with signs of exocytosis were observed in the aortic root, the large coronary arteries of the epicardium, the fibrous ring, the valves and the subaortal cone. vWF+ was less pronounced in the endothelium lining the atrial and ventricular cavities and in the myocardial capillaries.
Conclusions. Using immunohistochemical detection of the vWF, the endothelium features of different parts of the rat heart were identified. Structural features due to increased secretion of vascular endothelial cells of the heart were revealed. Further research in this direction may be important to explain the mechanisms and diagnosis of endothelial dysfunction.
About the Authors
E. I. Chumasov
Institute of Experimental Medicine; Saint-Petersburg State Academy of Veterinary Medicine
Russian Federation
Russian Federation
Chumasov Evgeny Ivanovich – professor, senior researcher, Laboratory of Functional Morphology of the Central and Peripheral Nervous System, Department of General and Special morphology
professor of the Department of Biology, Ecology and Histology
E. S. Petrova
Institute of Experimental Medicine
Russian Federation
Petrova Elena Sergeevna – PhD, senior researcher, Laboratory of Functional Morphology of the Central and Peripheral Nervous System, Department of General and Special morphology
Russian Federation
Petrova Elena Sergeevna – PhD, senior researcher, Laboratory of Functional Morphology of the Central and Peripheral Nervous System, Department of General and Special morphology
D. E. Korzhevskii
Institute of Experimental Medicine
Russian Federation
Korzhevskii Dmitrii Eduardovich – Doctor of Medicine (MD) in Histology and Cytology; Professor of the Russian Academy of Sciences, Chef of the Laboratory of Functional Morphology of the Central and Peripheral Nervous System, Department of General and Special morphology
Russian Federation
Korzhevskii Dmitrii Eduardovich – Doctor of Medicine (MD) in Histology and Cytology; Professor of the Russian Academy of Sciences, Chef of the Laboratory of Functional Morphology of the Central and Peripheral Nervous System, Department of General and Special morphology
References
1. Банин В. В. Роль внеклеточного матрикса в регуляции ангиогенеза // Регионарное кровообращение и микроциркуляция. – 2006. – Т. 5, № 1. – С. 13–19. [Banin VV. Rol’ vnekletochnogo matriksa v reguljacii angiogeneza. Regionarnoe krovoobrawenie i mikrocirkuljacija. 2006;5(1): 13–19. (In Russ.)].
2. Васина Л. В., Власов Т. Д., Петрищев Н. Н. Функциональная гетерогенность эндотелия (обзор) // Артериальная гипертензия. – 2017. – Т. 23, № 2. – С. 88–102. [Vasina LV, Vlasov TD, Petriwev NN. Funkcional’naja geterogennost’ jendotelija (obzor). Arterial’naja gipertenzija. 2017;23(2):88–102. (In Russ.)]. Doi: doi.org/10.18705/1607- 419X-2017-23-2-88-102.
3. Коржевский Д. Э., Кирик О. В., Сухорукова Е. Г. и др. Фактор Виллебранда эндотелиоцитов кровеносных сосудов и его использование в иммуноморфологических исследованиях // Мед. академ. журн. – 2017. – Т. 17, № 1. – С. 34–40. [Korzhevskij DJE., Kirik OV., Suhorukova EG., Alekseeva OS., SHajdakov EV. Faktor Villebranda jendoteliocitov krovenosnyh sosudov i ego ispol’zovanie v immunomorfologicheskih issledovanijahe. Medicinskij akademicheskij zhurnal. 2017;17(1):34–40. (In Russ.)].
4. Чумасов Е. И., Петрова Е. С., Коржевский Д. Э. Структурно-функциональная характеристика эндотелиальных клеток сосудов сердца новорожденной крысы (иммунногистохимическое исследование) // Регионарное кровообращение и микроциркуляция. – 2018. – Т. 17, № 2. – С. 80–85. [CHumasov EI, Petrova ES, Korzhevskij DJE. Strukturno-funkcional’naja harakteristika jendotelial’nyh kletok sosudov serdca novorozhdennoj krysy (immunnogistohimicheskoe issledovanie). Regionarnoe krovoobrawenie i mikrocirkuljacija. 2018;17(2):80–85. (In Russ.)]. Doi: 10.24884/1682-6655-2018-17-2-78-83.
5. Шабров А. В., Апресян А. Г., Добкес А. Л. и др. Роль и методы оценки эндотелиальной дисфункции в практической медицине // Мед. академ. журн. – 2017. – Т. 17, № 1. – С. 7–23. [Shabrov AV, Apresjan AG, Dobkes AL, Ermolov SJU, Ermolova TV, Manasjan SG, Serdjukov SV. Rol’ i metody ocenki jendotelial’noj disfunkcii v prakticheskoj medicine. Medicinskij akademicheskij zhurnal. 2017;17(1):7–23. (In Russ.)].
6. Петрищев Н. Н. Дисфункция эндотелия: патогенетическое значение и методы коррекции. – СПб.: СПб ИИЦ ВМА, 2007. – 269 с. [Petriwev NN. Disfunkcija jendotelija. Patogeneticheskoe znachenie i metody korrekcii. SPb., SPb IIC VMA, 2007:269. (In Russ.)].
7. Сухорукова Е. Г., Кирик О. В., Коржевский Д. Э. Выявление телец Вейбеля-Паладе при помощи иммуноцитохимической реакции на фактор Виллебранда и конфокальной лазерной микроскопии // Морфология. – 2018. – Т. 153, № 1. – С. 71–75. [Suhorukova EG, Kirik OV, Korzhevskij DJE. Vyjavlenie telec Vejbelja-Palade pri pomowi immunocitohimicheskoj reakcii na faktor Villebranda i konfokal’noj lazernoj mikroskopii. Morfologija. 2018;153(1):71–75. (In Russ.)].
8. Шайдаков Е. В., Евлахов В. И. Роль эндотелия в патогенезе хронической постэмболической легочной гипертензии // Ангиол. и сосуд. хир. – 2016. – Т. 22, № 1. – С. 22– 27. [Shajdakov EV, Evlahov VI. Rol’ jendotelija v patogeneze hronicheskoj postjemboli cheskoj ljogochnoj gipertenzii. Angiologija i sosudistaja hirurgija. 2016;22(1):22–27. (In Russ.)].
9. Babich V, Knipe L, Hewlett L, Meli A, Dempster J, Hannah MJ, Carter T. Differential effect of extracellular acidosis on the release and dispersal of soluble and membrane proteins secreted from the Weibel-Palade body. J. Biol. Chem. 2009;284(18):12459–12468. Doi: 10.1074/jbc.M809235200.
10. Michaux G, Cutler DF. How to roll endothelial cigar: the biogenesis of Weibel-Palade bodies. Traffic. 2004;5(2):69–78.
11. Valentijn KM, van Driel LF, Mourik MJ. Multigranular exocytosis of Weibel-Palade bodies in vascular endothelial cells. Blood. 2010;116(10):1807–1816. Doi: 10.1182/blood2010-03-274209.
12. Wagner DD, Bonfanti R. von Willebrand factor and the endothelium. Mayo Clin. Proc. 1991;66(6):621–627.
13. Weibel ER, Palade GE. New cytoplasmic components in arterial endothelia. J. Cell Biol. 1964;(23):101–112.
14. Коржевский Д. Э., Кирик О. В., Петрова Е. С. и др. Теоретические основы и практическое применение методов иммуногистохимии / под ред Д. Э. Коржевского. – 2-е изд., испр. и доп. СПб., 2014. – 119 с. [Korzhevskij DJE, Kirik OV, Petrova ES, Karpenko MN, Grigor’ev IP, Suhorukova EG, Kolos EA, Giljarov AV. Teoreticheskie osnovy i prakticheskoe primenenie metodov immunogistohimii / pod red D. Je. Korzhevskogo. 2-e izdanie, ispravlennoe i dopolnennoe. SPb., 2014:119. (In Russ.)].
15. Nightingale N, Cutler D. The secretion von Willebrand factor from endothelial cells; an increasingly complicated story. J. Thromb. Haemost. 2013;11(1):192–201. Doi: doi:10.1111/jth.12225.
16. Richardson M, Tinlin S, De Reske M. Morphological alterations in endothelial cells associated with the release of von Willebrand factor after thrombin generation in vivo. Arterioscler . Thromb. 1994;14(6):990–999.
17. Weibel ER. Fifty years of Weibel–Palade bodies: the discovery and early history of an enigmatic organelle of endothelial cells. J. Thromb. Haemost. 2012;10:979–984. Doi: doi:10.1111/j.1538-7836.2012.04718.x.
18. Huang RH, Wang Y, Roth R, Yu X, Purvis AR, Heuser JE, Egelman EH, Sadler JE. Assembly of Weibel–Palade body-like tubules from N–terminal domains of von Willebrand factor. Proc. Natl. Acad. Sci USA. 2008;105(2):482–487. Doi: doi:10.1073/pnas.0710079105.
19. Boneu B, Abbal M, Plante J, Bierme R. Letter: Factor-VIII complex and endothelial damage. Lancet. 1975; 1(7922):1430.
20. Fujimoto S. Degranulation of endothelial specific granules of the toad aorta after treatment with compound 48/80. Anat. Rec. 1982;203(2):197–204. Doi: 10.1002/ar. 1092030202.
21. Kumar RA, Moake JL, Nolasco L, Bergeron AL, Sun C, Dong JF, McIntire LV. Enhanced platelet adhesion and aggregation by endothelial cell-derived unusually large multimers of von Willebrand factor. Biorheology. Biorheology. 2006;43(5):681–691.
Review
For citations:
Chumasov E.I., Petrova E.S., Korzhevskii D.E. Structural and functional peculiarities of the endothelium of heart vessels of mature rats according to immunistochemical studies. Regional blood circulation and microcirculation. 2019;18(2):70-77. (In Russ.) https://doi.org/10.24884/1682-6655-2019-18-2-70-77
Views:
1461
Email this article 
