Catecholaminergic structures of the rat forebrain following transitory ischemia
https://doi.org/10.24884/1682-6655-2012-11-2-57-60
Abstract
Forebrain catecholaminergic system was studied in rats two days following focal ischemia induced by 30-min occlusion of middle brain artery. Catecholaminergic structures were revealed using immunocytochemical staining for tyrosine hydroxylase (TH), the enzyme of catecholamines synthesis. Distribution of TH is described in the forebrain areas in control - predominantly in fibers and terminals as well as some neurons of neocortex and striatum. Intensity of TH immunostaining reduced sharply in the brain artery occlusion hemisphere 2 days after ischemia. Probably, this is a result of increased reactivity to ischemia of catecholaminergic neurons of midbrain and brainstem projecting to the forebrain areas.
About the Authors
I. P. Grigorev
Institute of Experimental Medicine of the Russian Academy of Medical Sciences, Saint Petersburg
Russian Federation
Russian Federation
E. S. Petrova
Institute of Experimental Medicine of the Russian Academy of Medical Sciences, Saint Petersburg
Russian Federation
Russian Federation
E. G. Gilerovich
Institute of Experimental Medicine of the Russian Academy of Medical Sciences, Saint Petersburg
Russian Federation
Russian Federation
T. D. Vlasov
Pavlov Saint Petersburg State Medical University
Russian Federation
Russian Federation
D. E. Korzhevskii
Institute of Experimental Medicine of the Russian Academy of Medical Sciences, Saint Petersburg
Russian Federation
Russian Federation
References
1. Баришполец, В. В. Структурно-функциональная организация дофаминергической системы головного мозга / В. В. Баришполец, Ю. О. Федотова, Н. С. Сапронов // Эксперимент. клин. фармакол. - 2009. - № 72 (3). - С. 44-49.
2. Григорьев, И. П. Применение различных антител к тирозингидроксилазе для изучения катехоламинергических систем головного мозга млекопитающих / И. П. Григорьев [и др.] // Морфология. - 2010. - № 138 (6). - С. 60-63.
3. Коржевский, Д. Э. Моделирование одностороннего ишемического повреждения нейронов стриатума с помощью непродолжительной окклюзии средней мозговой артерии / Д. Э. Коржевский [и др.] // Бюлл. эксперимент. биол. мед. - 2009. - № 147 (2). - С. 217-219.
4. Коржевский, Д. Э. Иммуноцитохимическое выявление нейронов головного мозга с помощью селективного маркера NeuN / Д. Э. Коржевский [и др.] // Морфология. - 2005. - № 128 (5). - С. 76-78.
5. Отеллин, В. А. Нигрострионигральная система / В. А. Отеллин, Э. Б. Арушанян. - М.: Медицина, 1989.
6. Cools, R. Role of dopamine in the motivational and cognitive control of behavior / R. Cools // Neuroscientist. - 2008. - № 14 (4). - Р. 381-395.
7. Crespi, F. Middle cerebral artery occlusion alters neurotransmitter activities in ipsilateral and contralateral rat brain regions: an ex vivo voltammetric study / F. Crespi, C. Pietra // Neurosci. Lett. - 1997. - № 230 (2). - Р. 77-80.
8. Fallon, J. H. Collateralization of monoamine neurons: mesotelencephalic dopamine projections to caudate, septum, andfrontal cortex / J. H. Fallon // J. Neurosci. - 1981. - № 1 (12). - Р. 1361-1368.
9. Paxinos, G. The rat brain in stereotaxic coordinates / G. Paxinos, C. Watson. - N.-Y.: Academic Press, 1998.
10. Uchida, H. Long-term changes in the ipsilateral substantia nigra after transient focal cerebral ischaemia in rats / H. Uchida [et al] // Int. J. Exp. Path. - 2010. - № 91. - Р. 256-266.
Review
For citations:
Grigorev I.P., Petrova E.S., Gilerovich E.G., Vlasov T.D., Korzhevskii D.E. Catecholaminergic structures of the rat forebrain following transitory ischemia. Regional blood circulation and microcirculation. 2012;11(2):57-60. (In Russ.) https://doi.org/10.24884/1682-6655-2012-11-2-57-60
Views:
441
Email this article 
