Circulating microRNA-208a level is elevated after myocardial ischemia-reperfusion in the rat
https://doi.org/10.24884/1682-6655-2012-11-2-66-71
Abstract
MicroRNA is usually defined as a class of small untranslated RNAs involved in the modulation of different processes within the cell by means of specific repression of mRNA translation and/or acceleration of its degradation. Tissue-specific profile of microRNA expression as well as existence of mechanisms of microRNA transport from the cells into the biological fluids might be considered as important prerequisites for the use of microRNA as a disease biomarker, including cardiac ischemia-reperfusion injury. It is known that microRNA-208a is expressed uniquely in cardiac myocytes and, thus, might serve as a promising biomarker of cardiac injury. In the present study, the levels of circulating microRNA-208a were determined with real-time PCR after 30-min regional myocardial ischemia followed by 90-min reperfusion in the rat. It was shown that microRNA-208a level was approximately 20-fold higher in the animals subjected to myocardial ischemia-reperfusion as compared to sham-treated animals. These data lend support for the idea that circulating microRNA-208a may play a role of highly specific biomarker for cardiac ischemic injury.
About the Authors
А. V. Fedorov
V. A. Almazov Federal Center of Heart, Blood and Endocrinology, Saint-Petersburg
Russian Federation
Russian Federation
S. М. Minasyan
V. A. Almazov Federal Center of Heart, Blood and Endocrinology, Saint-Petersburg; I. P. Pavlov State Medical University
Russian Federation
Russian Federation
А. А. Kostareva
V. A. Almazov Federal Center of Heart, Blood and Endocrinology, Saint-Petersburg; I. P. Pavlov State Medical University
Russian Federation
Russian Federation
V. O. Kabanov
V. A. Almazov Federal Center of Heart, Blood and Endocrinology, Saint-Petersburg
Russian Federation
Russian Federation
М. М. Galagudza
V. A. Almazov Federal Center of Heart, Blood and Endocrinology, Saint-Petersburg; I. P. Pavlov State Medical University
Russian Federation
Russian Federation
D. I. Kurapeev
V. A. Almazov Federal Center of Heart, Blood and Endocrinology, Saint-Petersburg
Russian Federation
Russian Federation
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Review
For citations:
Fedorov А.V., Minasyan S.М., Kostareva А.А., Kabanov V.O., Galagudza М.М., Kurapeev D.I. Circulating microRNA-208a level is elevated after myocardial ischemia-reperfusion in the rat. Regional blood circulation and microcirculation. 2012;11(2):66-71. (In Russ.) https://doi.org/10.24884/1682-6655-2012-11-2-66-71
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