Oxidative stress in patients with intravascular coagulation disturbance

Abstract

Vascular pathology is closely associated with aging, oxidative stress, and endothelial or mitochondrial dysfunctions. The aim of the study was to assess whether the oxidant/antioxidant status of blood plasma changes in patients with activation of intravascular coagulation and fibrinolysis (AIVCF) in aspects of aging. We studied the specimens of blood plasma of 109 patients [76 males and 33 females; median age 52 (QIR 37-67) with AIVCF and d-dimer level >500 uL and 30 healthy controls]. The concentration of thiobarbituric acid reactive substances (TBARS) was higher in patients [8.5 (QIR 6.5-10.6) umol/L vs. 6.8 (QIR 5.8-7.4) umol/L; respectively (P<0.001)], SH-groups level was lower [106.4 (84.8-140.8)) umol/L vs. 168.2 (QIR 140.2-214.3) umol/L], and superoxide dismutase (SOD) activity was lower [7.6 (QIR 5.8-12.4) U/ml vs. 17.1 (QIR 13.9-19.6) U/ml; respectively; P=0.001] when compared with the healthy controls. When all of the patients were divided into two age-related groups: Group 1 [34 (QIR 28-45) years] and Group 2 [67 (QIR 65-75) years], we estimated a significant negative correlation (r= -0.528, P=0.01) between TBARS and SOD in Group 2. Plasma lactate level in elderly patients (Group 2) did not differ from those in Group 1, although it was significantly higher in the both groups when compared with the control group. We presume that AIVCF is accompanied by oxidative stress and lactacidemia independently of age. Appearance markers of oxidative stress (TBARP and SOD activity in plasma) and lactacidemia may be used for case study development and new therapeutic team approach to the AIVCF.