Adaptation to intermittent hypoxia prevents the decrease in cerebral vascular density in rats with experimental Alzheimer’s disease
https://doi.org/10.24884/1682-6655-2021-20-2-59-64
Abstract
Introduction. Patients with Alzheimer’s disease (AD) have reduced cerebral vascular density (VD), which impairs blood flow to neurons and may contribute to progression of AD. Earlier we showed that prior adaptation to intermittent hypobaric hypoxia (IHH) prevented memory loss and degeneration of cortical neurons in rats with experimental AD (EAD).
The aim of this study was to test if IHH might prevent EAD-induced vascular rarefaction in rats.
Materials and methods. EAD was induced with bilateral injection of neurotoxic beta-amyloid peptide fragment (A) (25–35) into n. basalis magnocellularis. IHH was simulated at a 4,000 m altitude, for 4 hours a day, for 14 days. Brain blood vessels were stained by transcardiac infusion of Indian ink; brain sections were stained with 0.3 % cresyl violet by Nissle method. Vascular density was assessed in the cortex and hippocampus using the Infinity Analysis Software.
Results. In the EAD rats, VD was significantly decreased in the hippocampus (13.3±0.9 vs 17.8±1.0 in field of view, FOV, p<0.03) and in the cortex (17.3±1.5 vs 22.3±1.3 in FOV, p<0.03). AIH increased VD in the hippocampus to 27.0±3.5 in FOV (p=0.01) and in cortex to 26.0±1.1 in FOV (p<0.03). In EAD+AIH rats, VD did not differ significantly from the control rats neither in the hippocampus, nor in the cortex. AIH may stimulate angiogenesis through hypoxia inducible factor-1α-mediated expression of vascular endothelial growth factor and/or by increasing expression and activity of antioxidant enzymes.
Conclusion. One of the mechanisms of AIH beneficial effect in AD-related neurodegeneration is preserving the capability for compensatory angiogenesis in brain.
About the Authors
A. V. Goryacheva
Institute of General Pathology and Pathophysiology
Russian Federation
Russian Federation
Goryacheva Anna V. – researcher
8, Baltiyskaya str., Moscow, 125315
I. V. Barskov
Moscow State University «Reaviz»
Russian Federation
Russian Federation
Barskov Igor V. – MD, associate Professor of the Department of Morphology and Pathology and head of Interdepartmental laboratory of Morphology
2, Krasnobogatyrskaya Str. Bldg. 2, Moscow, 107564
H. F. Downey
University of North Texas Health Science Center; South Ural State University
United States
United States
Downey Harry Fred – PhD, Reagents Professor; Consultant
3500 Camp Bowie Blvd, Fort Worth 76107
76, Lenina pr., Chelyabinsk, 454080
Eu. B. Manukhina
Institute of General Pathology and Pathophysiology; University of North Texas Health Science Center; South Ural State University
Russian Federation
Russian Federation
Manukhina Eugenia B., PhD, DSc, Professor, Head Researcher; Leading Researcher; Adjunct Professor
8, Baltiyskaya str., Moscow, 125315
3500 Camp Bowie Blvd, Fort Worth 76107, USA
76, Lenina pr., Chelyabinsk, 454080
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For citations:
Goryacheva A.V., Barskov I.V., Downey H.F., Manukhina E.B. Adaptation to intermittent hypoxia prevents the decrease in cerebral vascular density in rats with experimental Alzheimer’s disease. Regional blood circulation and microcirculation. 2021;20(2):59-64. (In Russ.) https://doi.org/10.24884/1682-6655-2021-20-2-59-64
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