The role of reactive oxygen species in the infarct-limiting effect of hypoxic preconditioning
https://doi.org/10.24884/1682-6655-2021-20-2-87-91
Abstract
Introduction. Increased resistance of the heart to ischemia/reperfusion (I/R) is an urgent aim of physiology, pharmacology, and cardiac surgery, since I/R injury of the heart is often the cause of cardiogenic shock and subsequent death of patients in the postoperative period.
Materials and methods. The study was carried out in male rats which were subjected to coronary artery occlusion (45 min) and reperfusion (2 h). Before coronary occlusion, early hypoxic preconditioning (HP) was modeled. The rats were subjected to six sessions of hypoxia (8 % O2, 10 min) and reoxygenation (21 % O2, 10 min) 30 min before coronary artery occlusion. The rats were injected with the following drugs: 1,3-dimethylthiourea (DMTM), 2-mercaptopropionyl glycine (2-MPG), deferoxamine.
Results. It was found that HP contributes to infarct size reduction by 30 %. Preliminary administration of DMTM, 2-MPG, deferoxamine eliminated the infarct-reducing effect of HP.
Conclisuon. The obtained data indicate that reactive oxygen species are involved in the cardioprotective effect of HP.
Supporting agencies: This article was prepared with the support of a grant from the Russian Foundation of Basic Research 21-515-53003. The section on deferoxamine is prepared within the framework of the state tasks AAAA-A15-115120910024-0. The authors are grateful to N. A. Danilchenko for technical assistance.
About the Authors
A. S. Sementsov
Cardiology Research Institute, Tomsk National Research Medical Center of the RAS; National Research Tomsk State University
Russian Federation
Russian Federation
Sementsov Andrey S. – Researcher, Laboratory of Experimental Cardiology; Assistant, Department of Physiology
111a, Kievskaya str., Tomsk, 634012
36, Lenin pr., Tomsk, 634050
N. V. Naryzhnaya
Cardiology Research Institute, Tomsk National Research Medical Center of the RAS
Russian Federation
Russian Federation
Naryzhnaya Natalia V. – MD, DSc, Principal Investigator, Laboratory of Experimental Cardiology
111a, Kievskaya str., Tomsk, 634012
M. A. Sirotina
Cardiology Research Institute, Tomsk National Research Medical Center of the RAS
Russian Federation
Russian Federation
Sirotina Maria A. – laboratory assistant, Laboratory of Experimental Cardiology
111a, Kievskaya str., Tomsk, 634012
L. N. Maslov
Cardiology Research Institute, Tomsk National Research Medical Center of the RAS
Russian Federation
Russian Federation
Maslov Leonid N. – MD, DSc, Professor, Head of the Laboratory of Experimental Cardiology
111a, Kievskaya str., Tomsk, 634012
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Review
For citations:
Sementsov A.S., Naryzhnaya N.V., Sirotina M.A., Maslov L.N. The role of reactive oxygen species in the infarct-limiting effect of hypoxic preconditioning. Regional blood circulation and microcirculation. 2021;20(2):87-91. (In Russ.) https://doi.org/10.24884/1682-6655-2021-20-2-87-91
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