A new protocol for isoheart rat kydney blood perfusion
https://doi.org/10.24884/1682-6655-2017-16-4-56-59
Abstract
Introduction and the purpose of the work. The perfusion of isolated laboratory animal organs is important for estimation of the effectiveness of methods elaborated for donor organ preservation. Materials and methods. Hemoperfusion of isolated rat kidney was performed after long-term hypothermal ischemia in order to reproduce reperfusion for the subsequent estimation of morphological changes in the cloth of nephritic parenchyma. Results. This method proved to be effective and technically simpler than different modifications of the classical procedure of kidney transplantation in small laboratory animals. Conclusions. The proposed procedure is an alternative to the classical kidney transplantation if reperfusion is required in an acute experiment for several hours.
About the Authors
S. M. Minasian
The Almazov National Medical Research Centre; Pavlov First Saint Petersburg State Medical University
Russian Federation
Russian Federation
A. E. Mamedov
Pavlov First Saint Petersburg State Medical University
Russian Federation
Russian Federation
Ja. I. Poleshenko
Pavlov First Saint Petersburg State Medical University
Russian Federation
Russian Federation
A. E. Scvortchov
Pavlov First Saint Petersburg State Medical University; Institute of Emergency Medicine
Russian Federation
Russian Federation
O. N. Reznik
Pavlov First Saint Petersburg State Medical University; Institute of Emergency Medicine
Russian Federation
Russian Federation
A. A. Kutenkov
Pavlov First Saint Petersburg State Medical University; Institute of Emergency Medicine
Russian Federation
Russian Federation
M. M. Galagudza
The Almazov National Medical Research Centre; Pavlov First Saint Petersburg State Medical University
Russian Federation
Russian Federation
D. L. Sonin
The Almazov National Medical Research Centre
Russian Federation
Russian Federation
A. A. Karpov
The Almazov National Medical Research Centre
Russian Federation
Russian Federation
O. M. Berko
Pavlov First Saint Petersburg State Medical University
Russian Federation
Russian Federation
Y. V. Dmitriev
The Almazov National Medical Research Centre
Russian Federation
Russian Federation
T. D. Vlasov
The Almazov National Medical Research Centre; Pavlov First Saint Petersburg State Medical University
Russian Federation
Russian Federation
References
1. Минасян С. М., Галагудза М. М., Дмитриев Ю. В. и др. Консервация донорского сердца: история и современность с позиции трансляционной медицины // Региональное кровообращение и микроциркуляция. - 2014. - Т. 13. - № 3 (51). - С. 4-16.
2. Резник О. Н. Оптимизация донорского ресурса трансплантации почек: организационный и технологический аспекты // Вестник трансплантологии и искусственных органов. - 2008. - № 1. - С. 14-22.
3. Shrestha B., Haylor J. Review of Surgical Techniques of Experimental Renal Transplantation in Rats // Experimental and Clinical Transplantation (2017) 4: 373-380.
4. Blom D., Orloff M. S. A more versatile and reliable method for renal transplantation in the rat. // Microsurgery. 1998;18(4):267-269.
5. Brown K., Phillips R. E., Wong W. What have we learnt from experimental renal transplantation? // Nephron Exp Nephrol. 2010; 115(1):e9-e14.
6. Karatzas T., Santiago S., Xanthos T., de Faria W., Gandia C., Kostakis A. An easy and safe model of kidney transplantation in rats. // Microsurgery. 2007; 27(8): 668-672.
7. Lopez-Neblina F., Toledo-Pereyra L. H., Suzuki S. Ultrarapid orthotopic technique for renal ransplantation in the rat. // Microsurgery. 1994; 15(4): 274-278.
8. Minasian S., Galagudza M., Dmitriev Yu., Karpov A., Vlasov T. Preservation of the donor heart: from basic science to clinical studies // Interact Cardiovasc Thorac Surg 2015 Apr 23; 20(4): 510-9. Epub 2014 Dec 23.
9. Schumacher M., Van Vliet B. N., Ferrari P. Kidney transplantation in rats: an appraisal of surgical techniques and outcome. // Microsurgery. 2003; 23(4): 387-394.
10. Shrestha B., Haylor J. Experimental rat models of chronic allograft nephropathy: a review. // Int J Nephrol Renovasc Dis. 2014; 7: 315-322.
Review
For citations:
Minasian S.M., Mamedov A.E., Poleshenko J.I., Scvortchov A.E., Reznik O.N., Kutenkov A.A., Galagudza M.M., Sonin D.L., Karpov A.A., Berko O.M., Dmitriev Y.V., Vlasov T.D. A new protocol for isoheart rat kydney blood perfusion. Regional blood circulation and microcirculation. 2017;16(4):56-59. (In Russ.) https://doi.org/10.24884/1682-6655-2017-16-4-56-59
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