The method of heterotopic rat heart transplantation for investigation the cardioprotective efficacy of cardioplegic solutions
https://doi.org/10.24884/1682-6655-2019-18-3-59-67
Abstract
This article describes in detail the modified method of heterotopic heart transplantation in rats. The methods of animal anesthesia, surgical features of the removal of a donor heart and its implantation to a recipient rat are described in detail. The advantages and disadvantages of donor heart preservation methods are described, as well as methods for evaluating a donor heart preparation, the quality of preservation and the effectiveness of cardioprotection. Experimental approaches to the study of the effectiveness of cardioplegic and preservative solutions using this technique are proposed. The data of our own pilot study on a comparative analysis of the effectiveness of cardioprotection using preservative solutions using this experimental model are presented.
Supporting agencies: The study is supported by the grant of the Russian Scientific Foundation No 17–04–02061 «MiR-223-5p and -3p-based myocardial necroptosis pathways in the cardiac allograft in heart transplantation».
About the Authors
S. M. Minasyan
Almazov National Medical Research Centre;
Pavlov University
Russian Federation
senior researcher at the Scientific Research Institute of Biomedicine of Pavlov University, senior researcher at the Scientific Research Laboratory of Microcirculation and Myocardial Metabolism of the Almazov National Medical Research Centre
Russian Federation
senior researcher at the Scientific Research Institute of Biomedicine of Pavlov University, senior researcher at the Scientific Research Laboratory of Microcirculation and Myocardial Metabolism of the Almazov National Medical Research Centre
Ya. I. Poleshenko
Almazov National Medical Research Centre;
Pavlov University
Russian Federation
student at the Pavlov University, Saint-Petersburg, zoological assistant of the Almazov National Medical Research Centre
Russian Federation
student at the Pavlov University, Saint-Petersburg, zoological assistant of the Almazov National Medical Research Centre
P. Yu. Shubina
Almazov National Medical Research Centre;
Pavlov University
Russian Federation
student at the Pavlov University, Saint-Petersburg, zoological assistant of the Almazov National Medical Research Centre
Russian Federation
student at the Pavlov University, Saint-Petersburg, zoological assistant of the Almazov National Medical Research Centre
E. S. Protsak
Almazov National Medical Research Centre;
Pavlov University
Russian Federation
student at the Pavlov University, Saint-Petersburg, zoological assistant of the Almazov National Medical Research Centre
Russian Federation
student at the Pavlov University, Saint-Petersburg, zoological assistant of the Almazov National Medical Research Centre
D. A. Druzhininsky
Pavlov University
Russian Federation
student
Russian Federation
student
M. M. Galagudza
Almazov National Medical Research Centre;
Pavlov University
Russian Federation
director at the Institute of Experimental Medicine of the Almazov National Medical Research Centre, professor at the Department of Pathophysiology of the Pavlov University, Saint-Petersburg, doctor of medical science, corresponding member of the Russian Academy of Sciences
Russian Federation
director at the Institute of Experimental Medicine of the Almazov National Medical Research Centre, professor at the Department of Pathophysiology of the Pavlov University, Saint-Petersburg, doctor of medical science, corresponding member of the Russian Academy of Sciences
I. S. Uskov
Almazov National Medical Research Centre
Russian Federation
junior researcher at the Institute of Experimental Medicine
Russian Federation
junior researcher at the Institute of Experimental Medicine
Yu. V. Dmitriev
Almazov National Medical Research Centre
Russian Federation
junior Researcher at the Institute of Experimental Medicine
Russian Federation
junior Researcher at the Institute of Experimental Medicine
References
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Review
For citations:
Minasyan S.M., Poleshenko Ya.I., Shubina P.Yu., Protsak E.S., Druzhininsky D.A., Galagudza M.M., Uskov I.S., Dmitriev Yu.V. The method of heterotopic rat heart transplantation for investigation the cardioprotective efficacy of cardioplegic solutions. Regional blood circulation and microcirculation. 2019;18(3):59-67. (In Russ.) https://doi.org/10.24884/1682-6655-2019-18-3-59-67
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