Non-invasive method of assessment of endothelium-derived hyperpolarizing factor in human
https://doi.org/10.24884/1682-6655-2013-12-4-70-74
Abstract
Endothelial hyperpolarizing factor (EDHF) currently remains the most poorly studied endothelial vasodilator. Evaluation of activity of EDHF can be done through other vazodilators «dual block» of the synthesis of nitric oxide and prostacyclin nitro-L-arginine and inhibitors of COX or, in contrast with, the block of the synthesis EDHF introduction tetraethylammonii chloride (TEA). Traditionally vasoactive substances blockers are injected intraarterially or intravenously producing systemic effect. We conducted a study in 30 healthy volunteers using ionophoretic administration of nitro-L-arginine inhibitor of COX (diclophenac) and TEA combined with ionophoresis of acetylcholine. The data obtained allow to conclude that both methods (block EDHF and block of NO-synthase activity plus COX) allow to evaluate the contribution of EDHF in endothelium-dependent vasodilation. Results obtained by applying these methods complement each other. Conclusion. Ionophoresis of blockers of vasoactive substances with the following measurement of dynamics of blood flow in the skin can be used to assess the contribution of EDHF in endothelium-dependent vasodilation.
About the Authors
Z. L. Malakhova
St. Petersburg Pavlov State Medical University
Russian Federation
Russian Federation
E. Yu. Vasina
St. Petersburg Pavlov State Medical University
Russian Federation
Russian Federation
E. A. Vorobiev
St. Petersburg Pavlov State Medical University
Russian Federation
Russian Federation
I. I. Nesterovich
St. Petersburg Pavlov State Medical University
Russian Federation
Russian Federation
T. D. Vlasov
St. Petersburg Pavlov State Medical University
Russian Federation
Russian Federation
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Review
For citations:
Malakhova Z.L., Vasina E.Yu., Vorobiev E.A., Nesterovich I.I., Vlasov T.D. Non-invasive method of assessment of endothelium-derived hyperpolarizing factor in human. Regional blood circulation and microcirculation. 2013;12(4):70-74. (In Russ.) https://doi.org/10.24884/1682-6655-2013-12-4-70-74
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